ArticleBMC genomics2018
Intramuscular preadipocytes impede differentiation and promote lipid deposition of muscle satellite cells in chickens.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 63 citations in OpenAlex.
- Integrated transcriptomic and metabolomic analyses reveal the regulatory mechanism of subcutaneous fat deposition in Baicheng Oil chickens.Poultry science · 2026Article
- Metabolic and cellular physiological differences between embryonic breast and leg muscle satellite cells in chickens.Poultry science · 2026Article
- Genomic Insights into Abdominal and Intramuscular Fat Deposition in Chickens and Their Implications for Productivity Traits: A Systematic Review.Animals : an open access journal from MDPI · 2026Review
- Bovine Muscle Satellite Cell-Derived Exosomes Modulate Preadipocyte Adipogenesis via bta-miR-2904.Animals : an open access journal from MDPI · 2026Article
- Bovine Adipocyte-Derived Exosomes Transport LncRNAs to Regulate Adipogenic Transdifferentiation of Bovine Muscle Satellite Cells.Animals : an open access journal from MDPI · 2025Article
- Molecular regulatory mechanisms of dietary supplementation with Allium mongolicum Regel powder to improve muscle development and meat quality in Angus calves.Animal bioscience · 2025Article
- The Molecular Mechanism by Which miR-211-5p Regulates the Proliferation and Differentiation of Preadipocytes in Meat Rabbits by Targeting TPK1.Animals : an open access journal from MDPI · 2025Article
- Transcriptomic Analysis of Muscle Satellite Cell Regulation on Intramuscular Preadipocyte Differentiation in Tan Sheep.International journal of molecular sciences · 2025Article
- Mature adipocytes inhibit differentiation of myogenic cells but stimulate proliferation of fibro-adipogenic precursors derived from trout muscle in vitro.Scientific reports · 2024Article
- RNA sequencing identifies key genes involved in intramuscular fat deposition in chickens at different developmental stages.BMC genomics · 2024Article
- Genomic Insights into Molecular Regulation Mechanisms of Intramuscular Fat Deposition in Chicken.Genes · 2023Review
- Possible Mechanisms Linking Obesity, Steroidogenesis, and Skeletal Muscle Dysfunction.Life (Basel, Switzerland) · 2023Review
- Comprehensive Proteome and Acetyl-Proteome Atlas Reveals Hepatic Lipid Metabolism in Layer Hens with Fatty Liver Hemorrhagic Syndrome.International journal of molecular sciences · 2023Article
- Towards a More Realistic In Vitro Meat: The Cross Talk between Adipose and Muscle Cells.International journal of molecular sciences · 2023Review
- Recent advances in the crosstalk between adipose, muscle and bone tissues in fish.Frontiers in endocrinology · 2023Review
- Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts.Journal of animal science and technology · 2022Article
- A selected population study reveals the biochemical mechanism of intramuscular fat deposition in chicken meat.Journal of animal science and biotechnology · 2022Article
- Comprehensive Transcriptome Sequencing Analysis ofFrontiers in physiology · 2022Article
- Transcriptional Insights into Key Genes and Pathways Underlying Muscovy Duck Subcutaneous Fat Deposition at Different Developmental Stages.Animals : an open access journal from MDPI · 2021Article
- MYOD1 inhibits avian adipocyte differentiation via miRNA-206/KLF4 axis.Journal of animal science and biotechnology · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundSkeletal muscle satellite cells (MSC) are crucial for postnatal growth and regeneration of skeletal muscle. An interaction exists between MSC and intramuscular preadipocytes (IMPA). This study is the first to investigate the effects of IMPA on MSC in chickens and unveil the molecular mechanisms by transcriptome analysis.
resultsPrimary MSC and IMPA were isolated from the pectoralis major muscle of 7-day-old chickens. After both cell types reached confluence, MSC were cultured alone or co-cultured with IMPA for 2 or 4 d. MSC treated for 2 d were subjected to RNA-seq. A total of 1653 known differentially expressed genes (DEG) were identified between co-cultured and mono-cultured MSC (|log2 FC| ≥ 1, FDR < 0.01). Based on Gene Ontology analysis, 48 DEG related to muscle development were screened, including the key genes MYOD1, MYOG, PAX7, and TMEM8C. The 44 DEG related to lipid deposition included the key genes CD36, FABP4, ACSBG2, CYP7A1 and PLIN2. Most of the DEG related to muscle development were downregulated in co-cultured MSC, and DEG related to lipid deposition were upregulated. Immunofluorescence of MHC supported IMPA impeding differentiation of MSC, and Oil Red O staining showed concurrent promotion of lipid deposition. Pathway analysis found that several key genes were enriched in JNK/MAPK and PPAR signaling, which may be the key pathways regulating differentiation and lipid deposition in MSC. Additionally, pathways related to cell junctions may also contribute to the effect of IMPA on MSC.
conclusionsThe present study showed that IMPA impeded differentiation of MSC while promoting their lipid deposition. Pathway analysis indicated that IMPA might inhibit differentiation via the JNK/MAPK pathway, and promote lipid deposition via the PPAR pathway. This study supplies insights into the effect of IMPA on MSC, providing new clues on exposing the molecular mechanisms underlying the interplay between skeletal muscle and intramuscular fat in chickens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.