ArticleBMC plant biology2018
Targeted mutagenesis using the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system in common wheat.
Article in BMC plant biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 108 citations in OpenAlex.
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- TaGα knockout in wheat causes early heading and short organ length, with dose-dependent effects through various pathways.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Genome editing‑based strategies to combat geminiviruses: CRISPR/Cas9 and emerging high‑fidelity tools.Archives of microbiology · 2026Review
- TaRIP2 Positively Regulates Wheat Pollen Wall Formation Through MYB80-Controlled Lipid Metabolism.Plant biotechnology journal · 2026Article
- Gene editing and association analysis of circadian clock gene TaPRR59 highlights its importance in yield-related traits in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- An NLR pair in the Pm68 locus confers powdery mildew resistance in durum and common wheat.Nature communications · 2025Article
- Harnessing primary, secondary and tertiary genepools for durable wheat disease resistance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Dehydrin Protein TaCOR410 Improves Drought Resistance of Wheat Through Autophagy.Plants (Basel, Switzerland) · 2025Article
- An Optimized Editing Approach for Wheat Genes by Improving sgRNA Design and Transformation Strategies.International journal of molecular sciences · 2025Article
- Creating a SuperiorFoods (Basel, Switzerland) · 2025Article
- CRISPR-based gene editing in plants: Focus on reagents and their delivery tools.BioImpacts : BI · 2025Review
- Comprehensive approaches to design efficient gRNA for SDN1-CRISPR/Cas9 genome editing in wheat.Frontiers in genome editing · 2025Article
- A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew.Nature communications · 2024Article
- Beyond pathways: Accelerated flavonoids candidate identification and novel exploration of enzymatic properties using combined mapping populations of wheat.Plant biotechnology journal · 2024Article
- The TaSnRK1-TabHLH489 module integrates brassinosteroid and sugar signalling to regulate the grain length in bread wheat.Plant biotechnology journal · 2024Article
- Wheat Pm55 alleles exhibit distinct interactions with an inhibitor to cause different powdery mildew resistance.Nature communications · 2024Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
backgroundRecently, the CRISPR/Cas9 system has been widely used to precisely edit plant genomes. Due to the difficulty in Agrobacterium-mediated genetic transformation of wheat, the reported applications in CRISPR/Cas9 system were all based on the biolistic transformation.
resultsIn the present study, we efficiently applied targeted mutagenesis in common wheat (Triticum aestivum L.) protoplasts and transgenic T0 plants using the CRISPR/Cas9 system delivered via Agrobacterium tumefaciens. Seven target sites in three genes (Pinb, waxy and DA1) were selected to construct individual expression vectors. The activities of the sgRNAs were evaluated by transforming the constructed vectors into wheat protoplasts. Mutations in the targets were detected by Illumina sequencing. Genome editing, including insertions or deletions at the target sites, was found in the wheat protoplast cells. The highest mutation efficiency was 6.8% in the DA1 gene. The CRISPR/Cas9 binary vector targeting the DA1 gene was then transformed into common wheat plants by Agrobacterium tumefaciens-mediated transformation, resulting in efficient target gene editing in the T0 generation. Thirteen mutant lines were generated, and the mutation efficiency was 54.17%. Mutations were found in the A and B genomes of the transgenic plants but not in the D genome. In addition, off-target mutations were not detected in regions that were highly homologous to the sgRNA sequences.
conclusionsOur results showed that our Agrobacterium-mediated CRISPR/Cas9 system can be used for targeted mutations and facilitated wheat genetic improvement.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.