ArticleNucleic acids research1988
The UAS of the yeast PGK gene is composed of multiple functional elements.
Article in Nucleic acids research, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Mediator binding to UASs is broadly uncoupled from transcription and cooperative with TFIID recruitment to promoters.The EMBO journal · 2016Article
- Understanding the growth phenotype of the yeast gcr1 mutant in terms of global genomic expression patterns.Journal of bacteriology · 2000Article
- In vivo analysis of functional regions within yeast Rap1p.Molecular and cellular biology · 1999Article
- Multiple domains of repressor activator protein 1 contribute to facilitated binding of glycolysis regulatory protein 1.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Activation mechanism of the multifunctional transcription factor repressor-activator protein 1 (Rap1p).Molecular and cellular biology · 1996Article
- The 3-phosphoglycerate kinase gene of the yeast Yarrowia lipolytica de-represses on gluconeogenic substrates.Current genetics · 1996Article
- The multifunctional transcription factors Abf1p, Rap1p and Reb1p are required for full transcriptional activation of the chromosomal PGK gene in Saccharomyces cerevisiae.Molecular & general genetics : MGG · 1996Article
- Control of glycolytic gene expression in the budding yeast (Saccharomyces cerevisiae).Current genetics · 1995Review
- The yeast protein Gcr1p binds to the PGK UAS and contributes to the activation of transcription of the PGK gene.Molecular & general genetics : MGG · 1994Article
- Expression of an endogenous and a heterologous gene in Candida maltosa by using a promoter of a newly-isolated phosphoglycerate kinase (PGK) gene.Current genetics · 1994Article
- A simple in vivo footprinting method to examine DNA-protein interactions over the yeast PYK UAS element.Nucleic acids research · 1994Article
- The yeast co-activator GAL11 positively influences transcription of the phosphoglycerate kinase gene, but only when RAP1 is bound to its upstream activation sequence.Molecular & general genetics : MGG · 1994Article
- Use of a selection technique to identify the diversity of binding sites for the yeast RAP1 transcription factor.Nucleic acids research · 1994Article
- GCR1, a transcriptional activator in Saccharomyces cerevisiae, complexes with RAP1 and can function without its DNA binding domain.The EMBO journal · 1993Article
- Stress response of yeast.The Biochemical journal · 1993Review
- Coordinated regulation and inositol-mediated and fatty acid-mediated repression of fatty acid synthase genes in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Role of GCR2 in transcriptional activation of yeast glycolytic genes.Molecular and cellular biology · 1992Article
- Characterization of the DNA-binding activity of GCR1: in vivo evidence for two GCR1-binding sites in the upstream activating sequence of TPI of Saccharomyces cerevisiae.Molecular and cellular biology · 1992Article
- A multi-component upstream activation sequence of the Saccharomyces cerevisiae glyceraldehyde-3-phosphate dehydrogenase gene promoter.Molecular & general genetics : MGG · 1991Article
- GCR1 of Saccharomyces cerevisiae encodes a DNA binding protein whose binding is abolished by mutations in the CTTCC sequence motif.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
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Abstract
The UAS (upstream activator sequence) of the yeast PGK gene contains a transcriptional activator domain located between bases -479 and -402 upstream from the initiating ATG. This region of the UAS contains three direct repeats of the sequence 5'CTTCC3'. The roles in transcriptional activation of these repeats and other sequences within the activator domain were investigated. When short regions containing the repeats were removed PGK expression was considerably reduced, the magnitude of the effect depended upon which CTTCC block was absent. Sequences between -473 and -458 which did not contain a CTTCC block were also shown to be necessary for high levels of PGK expression. A DNA fragment containing activator sequences up to -473 was shown to interact specifically in vitro with a yeast nuclear protein extract. DNase I footprinting identified a protected region between -473 and -458 and single base changes in DNase I sensitivity at the CTTCC repeats.
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