Evidence map›Paper›PMID 30476689›Full record

ReviewCurrent opinion in chemical biology2019

Protein identification strategies in MALDI imaging mass spectrometry: a brief review.

Daniel J Ryan, Jeffrey M Spraggins, Richard M Caprioli

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Current opinion in chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.

0numbers the graph read from it
0cells of the map it votes in
84citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

84 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. MALDI spatial proteomics: a mini review of approaches and techniques.Analytical methods : advancing methods and applications · 2026
    Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Review
  11. Review
  12. Review
  13. Article
  14. Cell-Instructive Biomaterials with Native-Like Biochemical Complexity.Annual review of biomedical engineering · 2025
    Review
  15. Article
  16. Article
  17. Article
  18. Review
  19. Mass spectrometry in animal health laboratories: recent history, current applications, and future directions.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024
    Review
  20. Article

24 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Daniel J RyanDepartment of Chemistry, Vanderbilt University, 7330 Stevenson Center, Station B 351822, Nashville, TN 37235, USA; Mass Spectrometry Research Center, Vanderbilt University, 465 21st Ave S #9160, Nashville, TN 37235, USA.
Jeffrey M SpragginsDepartment of Chemistry, Vanderbilt University, 7330 Stevenson Center, Station B 351822, Nashville, TN 37235, USA; Mass Spectrometry Research Center, Vanderbilt University, 465 21st Ave S #9160, Nashville, TN 37235, USA; Department of Biochemistry, Vanderbilt University, 607 Light Hall, Nashville, TN 37205, USA.
Richard M CaprioliDepartment of Chemistry, Vanderbilt University, 7330 Stevenson Center, Station B 351822, Nashville, TN 37235, USA; Mass Spectrometry Research Center, Vanderbilt University, 465 21st Ave S #9160, Nashville, TN 37235, USA; Department of Biochemistry, Vanderbilt University, 607 Light Hall, Nashville, TN 37205, USA; Department of Pharmacology, Vanderbilt University, 442 Robinson Research Building, 2220 Pierce Avenue, Nashville, TN 37232, USA; Department of Medicine, Vanderbilt University, 465 21st Ave S #9160, Nashville, TN 37235, USA. Electronic address: richard.m.caprioli@Vanderbilt.Edu.

Funding

Training (pp 214-245)P41GM103391 · NIGMS · VANDERBILT UNIVERSITY · PI CAPRIOLI, RICHARD M · 2012 to 2020
$17.7M
Vanderbilt University Biomolecular Multimodal Imaging Center for 3-Dimensional Tissue MappingU54DK120058 · NIDDK · VANDERBILT UNIVERSITY · PI HARRIS, RAYMOND C. · 2018 to 2021
$5.7M
NIDDK NIH HHS U54 DK120058NIGMS NIH HHS P41 GM103391
6 · The paper itself

Abstract

Matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a powerful technology used to investigate the spatial distributions of thousands of molecules throughout a tissue section from a single experiment. As proteins represent an important group of functional molecules in tissue and cells, the imaging of proteins has been an important point of focus in the development of IMS technologies and methods. Protein identification is crucial for the biological contextualization of molecular imaging data. However, gas-phase fragmentation efficiency of MALDI generated proteins presents significant challenges, making protein identification directly from tissue difficult. This review highlights methods and technologies specifically related to protein identification that have been developed to overcome these challenges in MALDI IMS experiments.

Indexed as

AnimalsEquipment DesignHumansMolecular ImagingProteinsSpecimen HandlingSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationWorkflowProteins

Identifiers

PMID30476689
PMCPMC6382520

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.