ArticlePloS one2018
Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Rickettsia massiliae and its public health significance across Palearctic and Oriental regions: a scoping review.Infectious diseases of poverty · 2026Article
- A rapid isothermal RPA-CRISPR/Cas12a assay for detection ofFrontiers in microbiology · 2026Article
- Hybrid paper/PDMS microfluidic device integrated with RNA extraction and recombinase polymerase amplification for detection of norovirus in foods.Applied and environmental microbiology · 2024Article
- Rocky Mountain Spotted Fever in Mexico: A Call to Action.The American journal of tropical medicine and hygiene · 2024Article
- Visual closed dumbbell-mediated isothermal amplification (CDA) for on-site detection of Rickettsia raoultii.PLoS neglected tropical diseases · 2022Article
- Recombinase polymerase amplification (RPA) with lateral flow detection for three Anaplasma species of importance to livestock health.Scientific reports · 2021Article
- Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay.Biosensors · 2021Article
- Rapid detection ofPeerJ · 2021Article
- Development of Rapid and Visual Nucleic Acid Detection Methods towards Four Serotypes of Human Adenovirus Species B Based on RPA-LF Test.BioMed research international · 2021Article
- Rapid detection of Burkholderia pseudomallei with a lateral flow recombinase polymerase amplification assay.PloS one · 2019Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesRickettsia rickettsii is the causative agent of Rocky Mountain spotted fever, which is the most severe spotted fever group (SFG) rickettsiosis. Developing a simple and reliable detection method is required.
methodsA detection method for R. rickettsii was established based on a recombinase polymerase amplification (RPA) assay and the lateral flow (LF) test. A specific target sequence was screened, and corresponding primers and probes were designed, synthesized, and screened for establishing an RPA assay with high amplification efficiency. Reagent concentrations, amplification time, and loading volume for strip development were optimized. The detection limit, analytic sensitivity and specificity were evaluated.
resultsA rapid, visual, sensitive and specific method for the detection of R. rickettsii based on RPA and the LF test was successfully established. The novel method had a limit of detection of 10 to 50 copies/reaction without recognizing other organisms. Analytical sensitivity and specificity were ≥90% and 100%, respectively, as evaluated by animal and simulative human samples.
conclusionsUsing the established method, detection could be completed in 30 min with visually detectable results by the naked eye, without requirement of any instrument except a constant temperature equipment. The technique shows superior detection performance and is promising for wide use in the field as well as resource-limited areas for R. rickettsii detection.
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