ArticlePloS one2018
Product authenticity versus globalisation-The Tulsi case.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Integrating DNA Barcoding Within an Orthogonal Approach for Herbal Product Authentication: A Narrative Review.Phytochemical analysis : PCA · 2025Review
- Monitoring Indian "Superfood" Moringa oleifera Lam. - species-specific PCR-fingerprint-based authentication for more consumer safety.NPJ science of food · 2024Article
- Development of an allele-specific PCR (AS-PCR) method for identifying high-methyl eugenol-containing purple Tulsi (Ocimum tenuiflorum L.) in market samples.Molecular biology reports · 2024Article
- Chloroplast genome ofMitochondrial DNA. Part B, Resources · 2024Article
- Review
- DNA barcoding as new diagnostic tool to lethal plant poisoning in herbivorous mammals.PloS one · 2023Article
- The Cultural and Commercial Value of Tulsi (Plants (Basel, Switzerland) · 2022Article
- Challenges in Medicinal and Aromatic Plants DNA Barcoding-Lessons from the Lamiaceae.Plants (Basel, Switzerland) · 2022Review
- Real-time qPCR for the detection of puffer fish components fromFrontiers in nutrition · 2022Article
- Composition of the metabolomic bio-coronas isolated from Ocimum sanctum and Rubia tinctorum.BMC research notes · 2021Article
- Cellular Base of Mint Allelopathy: Menthone Affects Plant Microtubules.Frontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using the Indian medicinal plant Tulsi (Holy Basil) as a case study, we have tested to what extent the discrepancy between vernacular and scientific nomenclature can be resolved, whether the presumed chemical diversity underlying the medicinal use of Tulsi has a genetic component, and whether it is possible to detect this genetic component using genetic barcoding markers. Based on four plastidic markers, we can define several haplotypes within Ocimum that are consistent across these markers. Haplotype II is congruent with O. tenuiflorum, while haplotype I extends over several members of the genus and cannot be resolved into genetically separate subclades. The vernacular subdivision of Tulsi into three types (Rama, Krishna, Vana) can only be partially linked with genetic differences-whereby Rama and Krishna Tulsi can be assigned to O. tenuiflorum, while Vana Tulsi belongs to haplotype I. This genetic difference is mirrored by differences in the profiles of secondary compounds. While developmental state and light quality modulate the amplitude to which the chemical profile is expressed, the profile itself seems to be linked with genetic differences. We finally develop an authentication assay that makes use of a characteristic single nucleotide polymorphism in one of the barcoding markers, establishing a differential restriction pattern that can be used to discriminate Vana Tulsi.
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