ArticleMolecular & general genetics : MGG1988
A consensus transcription termination sequence in the promoter region is necessary for efficient gene expression of the TRP1 gene of Saccharomyces cerevisiae.
Article in Molecular & general genetics : MGG, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 17 citations in OpenAlex.
- The requirement of yeast replication origins for pre-replication complex proteins is modulated by transcription.Nucleic acids research · 2005Article
- Genetic analysis of serine biosynthesis and glucose repression in yeast.Current genetics · 1992Article
- Multiple-copy integration of the alpha-galactosidase gene from Cyamopsis tetragonoloba into the ribosomal DNA of Kluyveromyces lactis.Current genetics · 1992Article
- Aromatic amino acid biosynthesis in the yeast Saccharomyces cerevisiae: a model system for the regulation of a eukaryotic biosynthetic pathway.Microbiological reviews · 1991Review
- DNA binding of CPF1 is required for optimal centromere function but not for maintaining methionine prototrophy in yeast.Nucleic acids research · 1991Article
- A GCN4 protein recognition element is not sufficient for GCN4-dependent regulation of transcription in the ARO7 promoter of Saccharomyces cerevisiae.Molecular & general genetics : MGG · 1990Article
- A 125-base-pair CEN6 DNA fragment is sufficient for complete meiotic and mitotic centromere functions in Saccharomyces cerevisiae.Molecular and cellular biology · 1989Article
- A single point mutation results in a constitutively activated and feedback-resistant chorismate mutase of Saccharomyces cerevisiae.Journal of bacteriology · 1989Article
- Cloning of the LEU2 gene of Saccharomyces cerevisiae by in vivo recombination.Archives of microbiology · 1989Article
- The REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis.Journal of bacteriology · 1989Article
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3 authors at 1 institution in 1 country.
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Abstract
The TRP1 gene of Saccharomyces cerevisiae is the only TRP gene which is not derepressible by the general control regulatory system. In the TRP1 promoter transcription starts at five initiation sites, organized in two clusters. The two transcripts of the first, more upstream cluster include a long leader sequence of approximately 200 bp. A transcriptional terminator element located in the 5' region of the TRP1 gene is essential for accurate gene expression. In partial TRP1 promoters lacking the terminator, like the original EcoRI TRP1 fragment used in numerous vectors, plasmid-encoded transcription is initiated predominantly in adjacent vector regions, resulting mainly in large, poorly translated transcripts. This poor translation is not due to mRNA instability. The effect can be suppressed by introducing artificial transcription barriers between vector sequences and the truncated EcoRI TRP1 fragment.
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