ArticleParasite (Paris, France)2018
Molecular characterization and protective efficacy of the microneme 2 protein from Eimeria tenella.
Article in Parasite (Paris, France), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Cecal invasion mediators EnMIC and its essential domain EnMAR2 confer immunoprotection against Eimeria necatrix in chickens.Poultry science · 2026Article
- Molecular characterization and immunoprotective potential of microneme protein 3 from Eimeria necatrix.Parasites & vectors · 2026Article
- Eimeria: Navigating complex intestinal ecosystems.PLoS pathogens · 2024Review
- Trends of Toxoplasma gondii and common transfusable venereal infections among blood donors in Menoufia Province, Egypt.Scientific reports · 2024Article
- Global profiling of protein S-palmitoylation in the second-generation merozoites of Eimeria tenella.Parasitology research · 2024Article
- Evaluation of the immune protective effects of rEmMIC2 and rEmMIC3 from Eimeria magna in rabbits.Parasitology research · 2023Article
- Recombinant Vaccinia Virus ExpressingTropical medicine and infectious disease · 2022Article
- Vaccines against chicken coccidiosis with particular reference to previous decade: progress, challenges, and opportunities.Parasitology research · 2022Review
- Identification and Protective Efficacy ofAnimals : an open access journal from MDPI · 2022Article
- Eimeria proteins: order amidst disorder.Parasites & vectors · 2022Review
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- Dual RNA-seq transcriptome analysis of caecal tissue during primary Eimeria tenella infection in chickens.BMC genomics · 2021Article
- Evaluation of 4 merozoite antigens as candidate vaccines against Eimeria tenella infection.Poultry science · 2021Article
- Specific EtMIC3-binding peptides inhibit Eimeria tenella sporozoites entry into host cells.Veterinary research · 2021Article
- Molecular characterization and protective efficacy of a new conserved hypothetical protein of Eimeria tenella.Parasite (Paris, France) · 2021Article
- Eimeria tenella Eimeria-specific protein that interacts with apical membrane antigen 1 (EtAMA1) is involved in host cell invasion.Parasites & vectors · 2020Article
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- Protective effects of a food-grade recombinant Lactobacillus plantarum with surface displayed AMA1 and EtMIC2 proteins of Eimeria tenella in broiler chickens.Microbial cell factories · 2020Article
- Further investigation of the characteristics and biological function of Eimeria tenella apical membrane antigen 1.Parasite (Paris, France) · 2020Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microneme proteins play an important role in the adherence of apicomplexan parasites to host cells during the invasion process. In this study, the microneme 2 protein from the protozoan parasite Eimeria tenella (EtMIC2) was cloned, characterized, and its protective efficacy as a DNA vaccine investigated. The EtMIC2 gene, which codes for a 35.07 kDa protein in E. tenella sporulated oocysts, was cloned and recombinant EtMIC2 protein (rEtMIC2) was produced in an Escherichia coli expression system. Immunostaining with an anti-rEtMIC2 antibody showed that the EtMIC2 protein mainly localized in the anterior region and membrane of sporozoites, in the cytoplasm of first- and second-generation merozoites, and was strongly expressed during first-stage schizogony. In addition, incubation with specific antibodies against EtMIC2 was found to efficiently reduce the ability of E. tenella sporozoites to invade host cells. Furthermore, animal-challenge experiments demonstrated that immunization with pcDNA3.1(+)-EtMIC2 significantly increased average body weight gain, while decreasing the mean lesion score and oocyst output in chickens. Taken together, these results suggest that EtMIC2 plays an important role in parasite cell invasion and may be a viable candidate for the development of new vaccines against E. tenella infection in chickens.
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