Evidence map›Paper›PMID 30473942›Full record

ArticleApplications in plant sciences2018

In silico detection of polymorphic microsatellites in the endangered Isis tamarind,

Joel Nichols, Gabriel C Conroy, Naga Kasinadhuni, Robert W Lamont, Steven M Ogbourne

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applications in plant sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Joel NicholsGeneCology Research Centre Faculty of Science Health, Engineering, and Education University of the Sunshine Coast Maroochydore DC Queensland 4558 Australia.
Gabriel C ConroyGeneCology Research Centre Faculty of Science Health, Engineering, and Education University of the Sunshine Coast Maroochydore DC Queensland 4558 Australia.
Naga KasinadhuniAustralian Genome Research Facility Level 5 Gehrmann Laboratories University of Queensland Research Road Brisbane Queensland 4072 Australia.
Robert W LamontGeneCology Research Centre Faculty of Science Health, Engineering, and Education University of the Sunshine Coast Maroochydore DC Queensland 4558 Australia.
Steven M OgbourneGeneCology Research Centre Faculty of Science Health, Engineering, and Education University of the Sunshine Coast Maroochydore DC Queensland 4558 Australia.ORCID 0000-0001-9812-4367

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

premise of the studyMETHODS AND

resultsWe used next-generation sequencing and bioinformatics to detect polymorphic microsatellites, in silico, reducing both the time and cost of marker development. A panel of 15 microsatellites, 12 of which were polymorphic, were subsequently characterized in 64 adult trees representing the entire species range. Mean observed heterozygosity and expected heterozygosity were 0.471 and 0.425, respectively. The polymorphism information content across loci ranged from 0.152 to 0.875.

conclusionsThe microsatellite markers developed in this study will be useful in gaining an understanding of

Indexed as

Alectryon ramiflorusconservation geneticsIllumina high‐throughput sequencingnext‐generation sequencing (NGS)reintroductionSapindaceae

Identifiers

PMID30473942
PMCPMC6240448

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.