ArticleMethods in cell biology2018
High-throughput microfluidic single-cell trapping arrays for biomolecular and imaging analysis.
Article in Methods in cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- DropBlot: single-cell western blotting of chemically fixed cancer cells.Nature communications · 2024Article
- Trapping of a Single Microparticle Using AC Dielectrophoresis Forces in a Microfluidic Chip.Micromachines · 2023Article
- Massively parallel quantification of phenotypic heterogeneity in single-cell drug responses.Science advances · 2021Article
- Image-Based Live Cell Sorting.Trends in biotechnology · 2021Review
- A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues.Frontiers in physics · 2021Article
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Authors and funding
2 authors.
Funding
Abstract
Single-cell analysis is of critical importance in revealing population heterogeneity, identifying minority sub-populations of interest, as well as discovering unique characteristics of individual cells. Microfluidic platforms work at the scale comparable to cell diameter and is suitable for single-cell manipulation. Here we present a microfluidic trapping array which is able to rapidly and deterministically trap single-cells in highly-packed microwells. This chapter first describes the design and fabrication protocols of the trapping array, and then presents its two representative applications: single-cell mRNA probing when integrated with a dielectrophoretic nanotweezer (DENT), and live-cell real-time imaging when combined with fluorescence lifetime imaging microscopy (FLIM). As the single-cell trapping efficiency is determined by the channel design instead of the flow rate, this trapping array can be coupled with different microfluidic sample processing units with different flow rates for various single-cell analyses.
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Registered trials
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