Evidence map›Paper›PMID 30473067›Full record

ArticleMethods in cell biology2018

Quantifying phenotypes in single cells using droplet microfluidics.

Fengjiao Lyu, Lucas R Blauch, Sindy K Y Tang

Abstract read
In one paragraph

Article in Methods in cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Fengjiao LyuDepartment of Mechanical Engineering, Stanford University, Stanford, CA, United States.
Lucas R BlauchDepartment of Mechanical Engineering, Stanford University, Stanford, CA, United States.
Sindy K Y TangDepartment of Mechanical Engineering, Stanford University, Stanford, CA, United States. Electronic address: sindy@stanford.edu.

Funding

Graduate Training Program in BiotechnologyT32GM008412 · NIGMS · STANFORD UNIVERSITY · PI COCHRAN, JENNIFER R · 1991 to 2020
$5.5M
NIGMS NIH HHS T32 GM008412
6 · The paper itself

Abstract

This book chapter describes the use of droplet microfluidics to phenotype single cells. The basic process flow includes the encapsulation of single cells with a specific probe into aqueous micro-droplets suspended in a biocompatible oil. The probe is chosen to measure the phenotype of interest. After incubation, the encapsulated cell turns the probe fluorescent and renders the entire droplet fluorescent. Enumerating drops that are fluorescent quantifies the concentration of cells possessing the phenotype of interest. Examining the distribution of fluorescence further allows one to quantify the heterogeneity among the cell population.

Indexed as

Microfluidic Analytical TechniquesEscherichia coliHumansPhenotypeSingle-Cell AnalysisTuberculosisDroplet microfluidicsExpressionHeterogeneityPhenotypeQuantificationSingle cell

Identifiers

PMID30473067
PMCPMC6880865

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.