ArticleiScience2018
Keratocytes Generate High Integrin Tension at the Trailing Edge to Mediate Rear De-adhesion during Rapid Cell Migration.
Article in iScience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Measuring cellular force using DNA-based tension probes: from ensemble to single-molecule studies.Nature protocols · 2026Review
- Cell migration in diabetic wound healing: Molecular mechanisms and therapeutic strategies (Review).International journal of molecular medicine · 2025Review
- The role and regulation of integrins in cell migration and invasion.Nature reviews. Molecular cell biology · 2025Review
- Analytical methods in studying cell force sensing: principles, current technologies and perspectives.Regenerative biomaterials · 2025Review
- Engineering tunable catch bonds with DNA.Nature communications · 2024Article
- Molecular Force Sensors for Biological Application.International journal of molecular sciences · 2024Review
- Unraveling the Dual-Stretch-Mode Impact on Tension Gauge Tethers' Mechanical Stability.Journal of the American Chemical Society · 2024Article
- Regulation of Cell Adhesion and Migration via Microtubule Cytoskeleton Organization, Cell Polarity, and Phosphoinositide Signaling.Biomolecules · 2023Review
- RAD-TGTs: high-throughput measurement of cellular mechanotype via rupture and delivery of DNA tension probes.Nature communications · 2023Article
- Tension Gauge Tethers as Tension Threshold and Duration Sensors.ACS sensors · 2023Article
- Vinculin transmits high-level integrin tensions that are dispensable for focal adhesion formation.Biophysical journal · 2023Article
- Single-Molecule Force Imaging Reveals That Podosome Formation Requires No Extracellular Integrin-Ligand Tensions or Interactions.ACS nano · 2022Article
- Characterization of Integrin Molecular Tension of Human Breast Cancer Cells on Anisotropic Nanopatterns.Frontiers in molecular biosciences · 2022Article
- A reversible shearing DNA probe for visualizing mechanically strong receptors in living cells.Nature cell biology · 2021Article
- Recent Advances in Cell Adhesive Force Microscopy.Sensors (Basel, Switzerland) · 2020Review
- Cell Migration Driven by Self-Generated Integrin Ligand Gradient on Ligand-Labile Surfaces.Current biology : CB · 2020Article
- Peptide nucleic acid based tension sensor for cellular force imaging with strong DNase resistance.Biosensors & bioelectronics · 2020Article
- Mechanical Regulation of Nuclear Translocation in Migratory Neurons.Frontiers in cell and developmental biology · 2020Review
- Exposure of the cryptic de-adhesive site FNIII14 in fibronectin molecule and its binding to membrane-type eEF1A induce migration and invasion of cancer cells via β1-integrin inactivation.American journal of cancer research · 2020Article
- DNA Nanotechnology as an Emerging Tool to Study Mechanotransduction in Living Systems.Small (Weinheim an der Bergstrasse, Germany) · 2019Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Rapid cell migration requires efficient rear de-adhesion. It remains undetermined whether cells mechanically detach or biochemically disassemble integrin-mediated rear adhesion sites in highly motile cells such as keratocytes. Using molecular tension sensor, we calibrated and mapped integrin tension in migrating keratocytes. Our experiments revealed that high-level integrin tension abbreviated as HIT, in the range of 50-100 pN (piconewton) and capable of rupturing integrin-ligand bonds, is exclusively and narrowly generated at cell rear margin during cell migration. Co-imaging of HIT and focal adhesions (FAs) shows that HIT is produced to mechanically peel off FAs that lag behind, and HIT intensity is correlated with the local cell retraction rate. High-level molecular tension was also consistently generated at the cell margin during artificially induced cell front retraction and during keratocyte migration mediated by biotin-streptavidin bonds. Collectively, these experiments provide direct evidence showing that migrating keratocytes concentrate force at the cell rear margin to mediate rear de-adhesion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.