ArticleScientific reports2018
Development a stacking pad design for enhancing the sensitivity of lateral flow immunoassay.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 99 citations in OpenAlex.
- Simplified Sample Preparation and Lateral Flow Immunoassay for the Detection of Plant Viruses.Biosensors · 2026Article
- Centrifugal Microfluidic Lateral Flow Assay Enables High Sensitivity Interleukin-6 Detection and Ultrafast Readout of Elevated Analyte Levels.Analytical chemistry · 2025Article
- Strategy for Accurate Detection of Six Tropane Alkaloids in Honey Using Lateral Flow Immunosensors.Sensors (Basel, Switzerland) · 2024Article
- Recent Advances in Lateral Flow Assays for Viral Protein Detection with Nanomaterial-Based Optical Sensors.Biosensors · 2024Review
- NT-proBNP detection with a one-step magnetic lateral flow channel assay.Analytical and bioanalytical chemistry · 2024Article
- Application of a Biomimetic Nanoparticle-Based Mock Virus to Determine SARS-CoV-2 Neutralizing Antibody Levels in Blood Samples Using a Lateral Flow Assay.Analytical chemistry · 2024Article
- Rapid detection ofMicrobiology spectrum · 2024Article
- PCR-Free Innovative Strategies for SARS-CoV-2 Detection.Advanced healthcare materials · 2023Review
- Clinical performance of SARS-CoV-2 antigen-detection rapid diagnostic test using SERS-based lateral flow immunoassay.Heliyon · 2023Article
- Engineering Innovative Interfaces for Point-of-Care Diagnostics.Current opinion in colloid & interface science · 2023Review
- Enhancing a SARS-CoV-2 nucleocapsid antigen test sensitivity with cost efficient strategy through a cotton intermembrane insertion.Scientific reports · 2023Article
- Economical and Easily Obtainable Tools to Manually Develop Lateral Flow Immunoassay Strips.ACS omega · 2023Article
- Clinical Evaluation of an Antigen Home Test Using Surface-Enhanced Raman Spectroscopy and Stacking Pad for SARS-CoV-2 Screening with Nasal and Salivary Swab Samples.Diagnostics (Basel, Switzerland) · 2023Article
- Nanotechnology-Enabled Biosensors: A Review of Fundamentals, Design Principles, Materials, and Applications.Biosensors · 2022Review
- Lateral Flow Immunoassays for Detecting Viral Infectious Antigens and Antibodies.Micromachines · 2022Review
- Lateral flow immunoassay for small-molecules detection in phytoproducts: a review.Journal of natural medicines · 2022Review
- Exploring carbohydrate binding module fusions and Fab fragments in a cellulose-based lateral flow immunoassay for detection of cystatin C.Scientific reports · 2022Article
- Optimizing Effective Parameters to Enhance the Sensitivity of Vertical Flow Assay for Detection ofBiosensors · 2022Article
- Amplified parallel antigen rapid test for point-of-care salivary detection of SARS-CoV-2 with improved sensitivity.Mikrochimica acta · 2021Article
- Disposable Paper-Based Biosensors for the Point-of-Care Detection of Hazardous Contaminations-A Review.Biosensors · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lateral flow immunoassays (LFIAs) have wide application in point-of-care testing, particularly in resource-poor settings. To achieve signal amplification in a gold nanoparticle-based lateral flow assay without an additional procedure or the need for complex fabrication, a new and simple method was developed for using a "stacking pad" configuration that adds an additional membrane between the conjugation pad and test pad to the conventional AuNP-based LFIA format. This design helps to accumulate the antibody and antigen on the stacking pad, hence extending the antigen/antibody binding interactions to enhance the test's detection sensitivity. With the enhanced lateral flow assay, as low as 1 ng/mL of Protein A and 15.5 ng/mL of C-reactive protein can be visualized with the naked eye. We also successfully applied the stacking pad system in the analysis of C-reactive protein in human serum and synovial fluid samples. These results suggest that this stacking pad LFIA can provide sensitive and on-site prognosis for detection in synovial fluid and serum samples in resource-limited settings.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.