ArticleScientific reports2018
Proof of concept study of mass cytometry in septic shock patients reveals novel immune alterations.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
- Integrated immune and endothelial profiling predicts 90-day mortality in postoperative sepsis and septic shock.EBioMedicine · 2026Observational
- Monocyte-macrophage membrane expression of IL-1R2 is a severity biomarker in sepsis.Cell death & disease · 2025Article
- The Predictive Value of Absolute Lymphocyte Count and T Cell Subpopulations for Sepsis Prognosis.Infection and drug resistance · 2024Article
- Dynamic immune signatures of patients with advanced non-small-cell lung cancer for infection prediction after immunotherapy.Frontiers in immunology · 2024Article
- Identifying dynamical persistent biomarker structures for rare events using modern integrative machine learning approach.Proteomics · 2023Article
- Prediction of outcome using CD14Medical microbiology and immunology · 2023Article
- Soluble CD4 effectively prevents excessive TLR activation of resident macrophages in the onset of sepsis.Signal transduction and targeted therapy · 2023Article
- Interrogating the sepsis host immune response using cytomics.Critical care (London, England) · 2023Review
- In-Depth Immunophenotyping With Mass Cytometry During TB Treatment Reveals New T-Cell Subsets Associated With Culture Conversion.Frontiers in immunology · 2022Article
- 4-Octyl itaconate regulates immune balance by activating Nrf2 and negatively regulating PD-L1 in a mouse model of sepsis.International journal of biological sciences · 2022Article
- Immune modulation after traumatic brain injury.Frontiers in medicine · 2022Review
- Changes in phenotypic patterns of blood monocytes after kidney transplantation and during acute rejection.Physiological research · 2021Article
- Plasma from patients with bacterial sepsis or severe COVID-19 induces suppressive myeloid cell production from hematopoietic progenitors in vitro.Science translational medicine · 2021Article
- Circulating mitochondrialProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Deciphering the state of immune silence in fatal COVID-19 patients.Nature communications · 2021Article
- Identification of stem cells from large cell populations with topological scoring.Molecular omics · 2021Article
- Assessing the Functional Heterogeneity of Monocytes in Human Septic Shock: a Proof-of-Concept Microfluidic Assay of TNFα Secretion.Frontiers in immunology · 2021Article
- Soluble PD-L1 improved direct ARDS by reducing monocyte-derived macrophages.Cell death & disease · 2020Article
- Deciphering heterogeneity of septic shock patients using immune functional assays: a proof of concept study.Scientific reports · 2020Article
- Immune Profiling Panel: A Proof-of-Concept Study of a New Multiplex Molecular Tool to Assess the Immune Status of Critically Ill Patients.The Journal of infectious diseases · 2020Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Innovative single cell technologies such as mass cytometry (CyTOF) widen possibilities to deeply improve characterisation of immune alterations mechanisms in human diseases. So far, CyTOF has not been used in sepsis - a condition characterized by complex immune disorders. Here, we evaluated feasibility of CyTOF analysis in patients with septic shock. We designed a mass cytometry panel of 25 extracellular markers to study mononuclear cells from 5 septic shock patients and 5 healthy donors. We explored single-cell data with global and specific unsupervised approaches such as heatmaps, SPADE and viSNE. We first validated relevance of our CyTOF results by highlighting established immune hallmarks of sepsis, such as decreased monocyte HLA-DR expression and increased expressions of PD1 and PD-L1 on CD4 T cells and monocytes. We then showed that CyTOF analysis reveals novel aspects of sepsis-induced immune alterations, e.g. B cell shift towards plasma cell differentiation and uniform response of several monocyte markers defining an immune signature in septic patients. This proof of concept study demonstrates CyTOF suitability to analyse immune features of septic patients. Mass cytometry could thus represent a powerful tool to identify novel pathophysiological mechanisms and therapeutic targets for immunotherapy in septic shock patients.
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