ArticleScientific reports2018
AutoFoci, an automated high-throughput foci detection approach for analyzing low-dose DNA double-strand break repair.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Repair of DNA double-strand breaks after low radiation doses in childhood cancer survivors and matched cancer-free individuals.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- An enhanced mountain climbing search algorithm to enable fast and accurate autofocusing in high resolution fluorescence microscopy.Methods and applications in fluorescence · 2025Article
- Foci-Xpress: Automated and Fast Nuclear Foci Counting Tool.International journal of molecular sciences · 2023Article
- Repair of α-particle-induced DNA damage in peripheral blood mononuclear cells after internal ex vivo irradiation withEuropean journal of nuclear medicine and molecular imaging · 2022Article
- HARLEY mitigates user bias and facilitates efficient quantification and co-localization analyses of foci in yeast fluorescence images.Scientific reports · 2022Article
- HiIDDD: a high-throughput imaging pipeline for the quantitative detection of DNA damage in primary human immune cells.Scientific reports · 2022Article
- DNA damage and repair in peripheral blood mononuclear cells after internal ex vivo irradiation of patient blood withEuropean journal of nuclear medicine and molecular imaging · 2022Article
- A deep learning model (FociRad) for automated detection of γ-H2AX foci and radiation dose estimation.Scientific reports · 2022Article
- Dosimetry of heavy ion exposure to human cells using nanoscopic imaging of double strand break repair protein clusters.Scientific reports · 2022Article
- Detection and quantification of γ-H2AX using a dissociation enhanced lanthanide fluorescence immunoassay.Scientific reports · 2021Article
- countfitteR: efficient selection of count distributions to assess DNA damage.Annals of translational medicine · 2021Article
- DeepFoci: Deep learning-based algorithm for fast automatic analysis of DNA double-strand break ionizing radiation-induced foci.Computational and structural biotechnology journal · 2021Article
- Review
- Dose and Dose-Rate Effects in a Mouse Model of Internal Exposure from 137Cs. Part 2: Integration of Gamma-H2AX and Gene Expression Biomarkers for Retrospective Radiation Biodosimetry.Radiation research · 2020Article
- DNA Damage in Blood Leukocytes of Prostate Cancer Patients Undergoing PET/CT Examinations with [Cancers · 2020Article
- Evaluation of machine learning models for automatic detection of DNA double strand breaks after irradiation using a γH2AX foci assay.PloS one · 2020Article
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Authors and funding
7 authors.
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Abstract
Double-strand breaks (DSBs) are the most lethal DNA damages induced by ionising radiation (IR) and their efficient repair is crucial to limit genomic instability. The cellular DSB response after low IR doses is of particular interest but its examination requires the analysis of high cell numbers. Here, we present an automated DSB quantification method based on the analysis of γH2AX and 53BP1 foci as markers for DSBs. We establish a combination of object properties, combined in the object evaluation parameter (OEP), which correlates with manual object classification. Strikingly, OEP histograms show a bi-modal distribution with two maxima and a minimum in between, which correlates with the manually determined transition between background signals and foci. We used algorithms to detect the minimum, thus separating foci from background signals and automatically assessing DSB levels. To demonstrate the validity of this method, we analyzed over 600.000 cells to verify results of previous studies showing that DSBs induced by low doses are less efficiently repaired compared with DSBs induced by higher doses. Thus, the automated foci counting method, called AutoFoci, provides a valuable tool for high-throughput image analysis of thousands of cells which will prove useful for many biological screening approaches.
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