ArticleSensors (Basel, Switzerland)2018
Cold Plasma as an Innovative Construction Method of Voltammetric Biosensor Based on Laccase.
Article in Sensors (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed.
- A Low-Cost and Environmentally Friendly Electrochemical Biosensor for the Determination of Estradiol.Materials (Basel, Switzerland) · 2025Article
- Fast Determination of Rutin on a Biosensor Made Using a Layered Double Hydroxide Nanocomposite Modified Electrode.Biosensors · 2023Article
- Biosensors Based on Phenol Oxidases (Laccase, Tyrosinase, and Their Mixture) for Estimating the Total Phenolic Index in Food-Related Samples.Life (Basel, Switzerland) · 2023Review
- MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin Determination.Biosensors · 2022Article
- Fabrication of a New, Low-Cost, and Environment-Friendly Laccase-Based Biosensor by Electrospray Immobilization with Unprecedented Reuse and Storage Performances.ACS sustainable chemistry & engineering · 2022Article
- Direct Exposure of Dry Enzymes to Atmospheric Pressure Non-Equilibrium Plasmas: The Case of Tyrosinase.Materials (Basel, Switzerland) · 2020Article
- Experimental Characterization of Polymer Surfaces Subject to Corona Discharges in Controlled Atmospheres.Polymers · 2019Article
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Authors and funding
5 authors.
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Abstract
Development of new, faster methods of biosensor construction is a huge challenge for current science and industry. In this work, biosensor construction was carried out using a new soft plasma polymerization (SPP) method in which a bio-recognition layer of laccase enzyme was polymerized and bonded to a glassy carbon electrode (GCE) substrate under atmospheric pressure with a corona discharge jet. Laccase belongs to the oxidoreductase enzyme group with four copper atoms in its active center. Application of the corona SPP plasma method allows reduction of the time needed for biosensor construction from several hours to minutes. The presented work includes optimization of the laccase bio-recognition layer deposition time, structural studies of the deposited laccase layer, as well as study of the fabricated biosensor applicability for the determination of Rutin in real pharmaceutical samples. This method produces a biosensor with two linear ranges from 0.3 μmol/dm³ to 0.5 μmol/dm³ and from 0.8 μmol/dm³ to 16 μmol/dm³ of Rutin concentration. Results shown in this work indicate that application of the one-step, corona SPP method enables biosensor construction with comparable analytical parameters to biosensors fabricated by conventional, multi-step, wet methods.
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