ArticleOrganic & biomolecular chemistry2019
Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA.
Article in Organic & biomolecular chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- High-throughput competitive binding assay for targeting RNA tertiary structures with small molecules: application to pseudoknots and G-quadruplexes.Nucleic acids research · 2025Article
- Design of deep-red emissive forced intercalation-induced light-up peptide as an indicator for the HIV-1 TAR RNA-ligand assay: integration of benzo[c,d]indole-quinoline (BIQ) cyanine dye into Tat peptide.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- Small molecule approaches to targeting RNA.Nature reviews. Chemistry · 2024Review
- Recognizing the power of machine learning and other computational methods to accelerate progress in small molecule targeting of RNA.RNA (New York, N.Y.) · 2023Article
- Method for Identifying Sequence Motifs in Pre-miRNAs for Small-Molecule Binding.ACS chemical biology · 2022Article
- A Fluorescent Reverse-Transcription Assay to Detect Chemical Adducts on RNA.Biochemistry · 2022Article
- Amilorides inhibit SARS-CoV-2 replication in vitro by targeting RNA structures.Science advances · 2021Article
- Combination of two analytical techniques improves wine classification by Vineyard, Region, and vintage.Food chemistry · 2021Article
- Small molecule targeting r(UGGAA)Nature communications · 2021Article
- Understanding the characteristics of nonspecific binding of drug-like compounds to canonical stem-loop RNAs and their implications for functional cellular assays.RNA (New York, N.Y.) · 2021Article
- Small molecule-RNA targeting: starting with the fundamentals.Chemical communications (Cambridge, England) · 2020Article
- Solid-phase fluorescent BODIPY-peptide synthesisChemical science · 2020Article
- IRES-targeting small molecule inhibits enterovirus 71 replication via allosteric stabilization of a ternary complex.Nature communications · 2020Article
- Target-Directed Approaches for Screening Small Molecules against RNA Targets.SLAS discovery : advancing life sciences R & D · 2020Review
- Template-guided selection of RNA ligands using imine-based dynamic combinatorial chemistry.Chemical communications (Cambridge, England) · 2020Article
- Driving factors in amiloride recognition of HIV RNA targets.Organic & biomolecular chemistry · 2019Article
- Methods to identify and optimize small molecules interacting with RNA (SMIRNAs).Drug discovery today · 2019Review
- Fluorescent indicator displacement assays to identify and characterize small molecule interactions with RNA.Methods (San Diego, Calif.) · 2019Review
- RNA Structural Differentiation: Opportunities with Pattern Recognition.Biochemistry · 2019Review
- Differentiation and classification of RNA motifs using small molecule-based pattern recognition.Methods in enzymology · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
A prominent hurdle in developing small molecule probes against RNA is the relative scarcity of general screening methods. In this study, we demonstrate the application of a fluorescent peptide displacement assay to screen small molecule probes against four different RNA targets. The designed experimental protocol combined with statistical analysis provides a fast and convenient method to simultaneously evaluate small molecule libraries against different RNA targets and classify them based on affinity and selectivity patterns.
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Registered trials
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