ArticlePlant biotechnology journal2019
Single-molecule real-time sequencing reveals diverse allelic variations in carotenoid biosynthetic genes in pepper (Capsicum spp.).
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- Laminar patterning transcription factors orchestrate spatial metabolite partitioning in Capsicum fruit.Nature plants · 2026Article
- Recent Advances in Pepper Fruit Glossiness.Genes · 2025Review
- Integrated metabolome and transcriptome analysis provides clues to fruit color formation of yellow, orange, and red bell pepper.Scientific reports · 2024Article
- Multi-omics analysis provides insights into the mechanism underlying fruit color formation inFrontiers in plant science · 2024Article
- Pigment Biosynthesis and Molecular Genetics of Fruit Color in Pepper.Plants (Basel, Switzerland) · 2023Review
- High-quality chromosome-scale genomes facilitate effective identification of large structural variations in hot and sweet peppers.Horticulture research · 2022Article
- Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation.PloS one · 2022Article
- Investigation of genetic factors regulating chlorophyll and carotenoid biosynthesis in red pepper fruit.Frontiers in plant science · 2022Article
- Nucleotide variation in the phytoene synthase (ClPsy1) gene contributes to golden flesh in watermelon (Citrullus lanatus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Identification of Compounds with Potential Therapeutic Uses from Sweet Pepper (International journal of molecular sciences · 2021Article
- The Genetic Components of a Natural Color Palette: A Comprehensive List of Carotenoid Pathway Mutations in Plants.Frontiers in plant science · 2021Review
- Chili Pepper Carotenoids: Nutraceutical Properties and Mechanisms of Action.Molecules (Basel, Switzerland) · 2020Review
- TaCKX gene family, at large, is associated with thousand-grain weight and plant height in common wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Phytoene synthase 2 can compensate for the absence of PSY1 in the control of color in Capsicum fruit.Journal of experimental botany · 2020Article
- Genetic mapping of the c1 locus by GBS-based BSA-seq revealed Pseudo-Response Regulator 2 as a candidate gene controlling pepper fruit color.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Development and Characterization of an Ethyl Methane Sulfonate (EMS) Induced Mutant Population inPlants (Basel, Switzerland) · 2020Article
- Candidate Gene Analysis Reveals That the Fruit Color LocusFrontiers in plant science · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diverse colours of mature pepper (Capsicum spp.) fruit result from the accumulation of different carotenoids. The carotenoid biosynthetic pathway has been well elucidated in Solanaceous plants, and analysis of candidate genes involved in this process has revealed variations in carotenoid biosynthetic genes in Capsicum spp. However, the allelic variations revealed by previous studies could not fully explain the variation in fruit colour in Capsicum spp. due to technical difficulties in detecting allelic variation in multiple candidate genes in numerous samples. In this study, we uncovered allelic variations in six carotenoid biosynthetic genes, including phytoene synthase (PSY1, PSY2), lycopene β-cyclase, β-carotene hydroxylase, zeaxanthin epoxidase and capsanthin-capsorubin synthase (CCS) genes, in 94 pepper accessions by single-molecule real-time (SMRT) sequencing. To investigate the relationship between allelic variations in the candidate genes and differences in fruit colour, we performed ultra-performance liquid chromatography analysis using 43 accessions representing each allelic variation. Different combinations of dysfunctional mutations in PSY1 and CCS could explain variation in the compositions and levels of carotenoids in the accessions examined in this study. Our results demonstrate that SMRT sequencing technology can be used to rapidly identify allelic variation in target genes in various germplasms. The newly identified allelic variants will be useful for pepper breeding and for further analysis of carotenoid biosynthesis pathways.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.