ArticlePlant biotechnology journal2019
Engineering 'designer' glycomodules for boosting recombinant protein secretion in tobacco hairy root culture and studying hydroxyproline-O-glycosylation process in plants.
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Protein engineering strategies to optimise recombinant product synthesis and accumulation in Nicotiana benthamiana.Plant biotechnology journal · 2026Review
- Advances in promoter engineering strategies for enhanced recombinant protein expression in plants.Frontiers in plant science · 2025Review
- Acidothermus cellulolyticus E1 endoglucanase expressed in planta undergoes extensive hydroxyproline-O-glycosylation and exhibits enhanced impact on biomass digestibility.Plant cell reports · 2024Article
- Intracellular trafficking and glycosylation of hydroxyproline-O-glycosylation module in tobacco BY-2 cells is dependent on medium composition and transcriptome analysis.Scientific reports · 2023Article
- Development of a tag-free plant-made interferon gamma production system with improved therapeutic efficacy against viruses.Frontiers in bioengineering and biotechnology · 2023Article
- Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants.Frontiers in plant science · 2023Review
- Collection of Hairy Roots as a Basis for Fundamental and Applied Research.Molecules (Basel, Switzerland) · 2022Review
- High yield secretion of human erythropoietin from tobacco cells for ex vivo differentiation of hematopoietic stem cells towards red blood cells.Journal of biotechnology · 2022Article
- Improving Protein Quantity and Quality-The Next Level of Plant Molecular Farming.International journal of molecular sciences · 2022Review
- Hairy roots: An untapped potential for production of plant products.Frontiers in plant science · 2022Review
- Plant cell-secreted stem cell factor stimulates expansion and differentiation of hematopoietic stem cells.Process biochemistry (Barking, London, England) · 2021Article
- Plant ProteinFrontiers in plant science · 2021Review
- A secreted protein of 15 kDa plays an important role in Phytophthora palmivora development and pathogenicity.Scientific reports · 2020Article
- Fusion of a Novel Native Signal Peptide Enhanced the Secretion and Solubility of Bioactive Human Interferon Gamma Glycoproteins inFrontiers in plant science · 2020Article
- On the Potential Function of Type II ArabinogalactanFrontiers in plant science · 2020Review
- High Efficient Expression and Purification of Human Epidermal Growth Factor inInternational journal of molecular sciences · 2019Article
- Unlocking the full potential of plant cell-based production for valuable proteins: Challenges and innovative strategies.Biotechnology advancesReview
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The key technical bottleneck for exploiting plant hairy root cultures as a robust bioproduction platform for therapeutic proteins has been low protein productivity, particularly low secreted protein yields. To address this, we engineered novel hydroxyproline (Hyp)-O-glycosylated peptides (HypGPs) into tobacco hairy roots to boost the extracellular secretion of fused proteins and to elucidate Hyp-O-glycosylation process of plant cell wall Hyp-rich glycoproteins. HypGPs representing two major types of cell wall glycoproteins were examined: an extensin module consisting of 18 tandem repeats of 'Ser-Hyp-Hyp-Hyp-Hyp' motif or (SP4)
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