Evidence map›Paper›PMID 30467667›Full record

ArticleMolecular biology reports2019

Curcumin prevented human autocrine growth hormone (GH) signaling mediated NF-κB activation and miR-183-96-182 cluster stimulated epithelial mesenchymal transition in T47D breast cancer cells.

Ajda Coker-Gurkan, Derya Bulut, Recep Genc, Elif-Damla Arisan, Pınar Obakan-Yerlikaya, Narcin Palavan-Unsal

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
1.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 46 citations in OpenAlex.

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  3. Cancer chemoprevention: signaling pathways and strategic approaches.Signal transduction and targeted therapy · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Ajda Coker-GurkanMolecular Biology and Genetics Department, Science and Letters Faculty, Istanbul Kultur University, Atakoy Campus, 34156, Istanbul, Turkey. a.coker@iku.edu.tr.ORCID http://orcid.org/0000-0003-1475-2417
Derya BulutMolecular Biology and Genetics Department, Science and Letters Faculty, Istanbul Kultur University, Atakoy Campus, 34156, Istanbul, Turkey.ORCID http://orcid.org/0000-0003-1903-6145
Recep GencMolecular Biology and Genetics Department, Science and Letters Faculty, Istanbul Kultur University, Atakoy Campus, 34156, Istanbul, Turkey.ORCID http://orcid.org/0000-0002-6872-2945
Elif-Damla ArisanMolecular Biology and Genetics Department, Science and Letters Faculty, Istanbul Kultur University, Atakoy Campus, 34156, Istanbul, Turkey.ORCID http://orcid.org/0000-0002-4844-6381
Pınar Obakan-YerlikayaMolecular Biology and Genetics Department, Science and Letters Faculty, Istanbul Kultur University, Atakoy Campus, 34156, Istanbul, Turkey.ORCID http://orcid.org/0000-0001-7058-955X
Narcin Palavan-UnsalMolecular Biology and Genetics Department, Science and Letters Faculty, Istanbul Kultur University, Atakoy Campus, 34156, Istanbul, Turkey.ORCID http://orcid.org/0000-0001-6366-6908
Istanbul Kültür University · TR

Funding

Scientific and Technological Research Council of TURKEY (TUBITAK), Grand Number: 113Z791 and Istanbul Kultur University Scientific Projects Support Center. 117Z791
6 · The paper itself

Abstract

Autocrine growth hormone (GH) signaling is a promoting factor for breast cancer via triggering abnormal cell growth, proliferation, and metastasis, drug resistance. Curcumin (diferuloylmethane), a polyphenol derived from turmeric (Curcuma longa), has anti-proliferative, anti-carcinogenic, anti-hormonal effect via acting on PI3K/Akt, NF-κB and JAK/STAT signaling. Forced GH expression induced epithelial mesenchymal transition (EMT) through stimulation of miR-182-96-183 cluster expression in breast cancer cells. This study aimed to investigate the role of NF-κB signaling and miR-182-96-183 cluster expression profile on autocrine GH-mediated curcumin resistance, which was prevented by time-dependent curcumin treatment in T47D breast cancer cells. Dose- and time-dependent effect of curcumin on T47D wt and GH+ breast cancer cells were evaluated by MTT cell viability and trypan blue assay. Apoptotic effect of curcumin was determined by PI and Annexin V/PI FACS flow analysis. Immunoblotting performed to investigate the effect of curcumin on PI3K/Akt/MAPK, NF-κB signaling. miR182-96-183 cluster expression profile was observed by qRT-PCR. Overexpression of GH triggered resistant profile against curcumin (20 µM) treatment for 24 h, but this resistance was accomplished following 48 h curcumin exposure. Concomitantly, forced GH induced invasion and metastasis through EMT and NF-κB activation were prevented by long-term curcumin exposure in T47D cells. Moreover, 48 h curcumin treatment prevented the autocrine GH-mediated miR-182-96-183 cluster expression stimulation in T47D cells. In consequence, curcumin treatment for 48 h, prevented autocrine GH-triggered invasion-metastasis, EMT activation through inhibiting NF-κB signaling and miR-182-96-183 cluster expression and induced apoptotic cell death by modulating Bcl-2 family members in T47D breast cancer cells.

Indexed as

ApoptosisAutocrine CommunicationBreast NeoplasmsCell Line, TumorCell MovementCell ProliferationCurcumaCurcuminEpithelial-Mesenchymal TransitionFemaleHuman Growth HormoneHumansMicroRNAsMitogen-Activated Protein KinasesNF-kappa BPhosphatidylinositol 3-KinasesCurcuminHuman Growth HormoneMicroRNAsMirn182 microRNA, humanMIRN183 microRNA, humanMIRN96 microRNA, humanMitogen-Activated Protein KinasesNF-kappa BPhosphatidylinositol 3-KinasesPlant ExtractsProto-Oncogene Proteins c-aktturmeric extractBreast cancerEpithelial mesenchymal transitionmiRNANF-κB

Identifiers

PMID30467667
OpenAlexW2901160393

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.