Evidence map›Paper›PMID 30467453›Full record

ArticleJournal of the World Aquaculture Society2018

Addressing Reproducibility in Cryopreservation, and Considerations Necessary for Commercialization and Community Development in Support of Genetic Resources of Aquatic Species.

Leticia Torres, Terrence R Tiersch

Abstract read
In one paragraph

Article in Journal of the World Aquaculture Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Use of Cryopreserved Sperm of Grass Carp (Aquaculture research · 2024
    Article
  5. Biopreservation in Agriculture and Food Systems: A Summary of Ethical Issues.The Journal of law, medicine & ethics : a journal of the American Society of Law, Medicine & Ethics · 2024
    Article
  6. Article
  7. The Journal of law, medicine & ethics : a journal of the American Society of Law, Medicine & Ethics · 2024
    Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Cryopreservation ofAnimals : an open access journal from MDPI · 2022
    Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. A generalized approach for sperm cryopreservation in the genusJournal of the World Aquaculture Society · 2021
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Leticia TorresAquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, USA 70820.
Terrence R TierschAquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, USA 70820.

Funding

Enhanced Development of the Xiphophorus Model System R24-OD-011120R24OD011120 · OD · TEXAS STATE UNIVERSITY · PI WALTER, RONALD B · 2012 to 2019
$5.3M
Development of Germplasm Resources for Preservation of Aquatic ModelsR24OD010441 · OD · LOUISIANA STATE UNIV AGRICULTURAL CENTER · PI BLACKBURN, HARVEY D., MONROE, WILLIAM · 2012 to 2021
$4.3M
NIH HHS R24 OD010441NIH HHS R24 OD011120
6 · The paper itself

Abstract

For the past six decades a repeated cycle of developing new cryopreservation protocols or simply reinventing them to counteract a lack of reproducibility has led to hundreds of published studies that have offered little to the establishment of a genetic resources community for aquatic species. This has hampered repository development and inhibited industrial application. Most protocols were developed without standardized approaches, leading to irreproducible studies and questionable or meaningless comparisons. Thus cryopreservation of germplasm in aquatic species would greatly benefit from strategies to facilitate reproducibility. Our objectives were to: (1) identify major sources of irreproducibility across research, small-scale, repository, and commercial-scale development levels, (2) provide recommendations to address reproducibility challenges, and (3) offer suggestions on how researchers can directly influence commercial development and application of cryopreservation research. Sources of irreproducibility include lack of standardized procedural approaches, lack of standardized terminology, and lack of reporting guidelines. To address these challenges, we propose implementation of standard operating procedures (SOP), support of stock centers and internet content for development of training programs, and strengthening of the role of scientific journals and reviewers in reducing the frequency of irreproducible outcomes. Reproducibility is the foundation for quality management programs and product reliability, and therefore standardization is necessary to assure efficient transition to commercial-scale application and repository development. Progress can only be possible through community-based approaches focused on coalescence and consensus of disparate groups involved in aquatic species cryopreservation and management of genetic resources.

Identifiers

PMID30467453
PMCPMC6241537

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.