ArticleScientific reports2018
Development of a multi-locus CRISPR gene drive system in budding yeast.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- CRISPR-Based genome editing in pulses: current approaches, challenges, and future prospects.Plant molecular biology · 2025Review
- Manipulating the Destiny of Wild Populations Using CRISPR.Annual review of genetics · 2023Review
- Rapid, scalable, combinatorial genome engineering by marker-less enrichment and recombination of genetically engineered loci in yeast.Cell reports methods · 2023Article
- Application progress of CRISPR/Cas9 genome-editing technology in edible fungi.Frontiers in microbiology · 2023Review
- Daisy-chain gene drives: The role of low cut-rate, resistance mutations, and maternal deposition.PLoS genetics · 2022Article
- A New Approach to Develop Resistant Cultivars Against the Plant Pathogens: CRISPR Drives.Frontiers in plant science · 2022Article
- Reflection on the Challenges, Accomplishments, and New Frontiers of Gene Drives.Biodesign research · 2022Review
- Analysis of a Cas12a-based gene-drive system in budding yeast.Access microbiology · 2021Article
- Multiplex Genome Editing in Yeast by CRISPR/Cas9 - A Potent and Agile Tool to Reconstruct Complex Metabolic Pathways.Frontiers in plant science · 2021Review
- CRISPR/Cas9 knockout of female-biased genes AeAct-4 or myo-fem in Ae. aegypti results in a flightless phenotype in female, but not male mosquitoes.PLoS neglected tropical diseases · 2020Article
- Advances in Molecular Tools and In Vivo Models for the Study of Human Fungal Pathogenesis.Microorganisms · 2020Review
- Assessment of a Split Homing Based Gene Drive for Efficient Knockout of Multiple Genes.G3 (Bethesda, Md.) · 2020Article
- CRISPR-Cas System: An Approach With Potentials for COVID-19 Diagnosis and Therapeutics.Frontiers in cellular and infection microbiology · 2020Review
- Advances in targeting and heterologous expression of genes involved in the synthesis of fungal secondary metabolites.RSC advances · 2019Review
- Keeping crispr in check: diverse mechanisms of phage-encoded anti-crisprs.FEMS microbiology letters · 2019Review
- Article
- Analysis of CRISPR gene drive design in budding yeast.Access microbiology · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The discovery of CRISPR/Cas gene editing has allowed for major advances in many biomedical disciplines and basic research. One arrangement of this biotechnology, a nuclease-based gene drive, can rapidly deliver a genetic element through a given population and studies in fungi and metazoans have demonstrated the success of such a system. This methodology has the potential to control biological populations and contribute to eradication of insect-borne diseases, agricultural pests, and invasive species. However, there remain challenges in the design, optimization, and implementation of gene drives including concerns regarding biosafety, containment, and control/inhibition. Given the numerous gene drive arrangements possible, there is a growing need for more advanced designs. In this study, we use budding yeast to develop an artificial multi-locus gene drive system. Our minimal setup requires only a single copy of S. pyogenes Cas9 and three guide RNAs to propagate three gene drives. We demonstrate how this system could be used for targeted allele replacement of native genes and to suppress NHEJ repair systems by modifying DNA Ligase IV. A multi-locus gene drive configuration provides an expanded suite of options for complex attributes including pathway redundancy, combatting evolved resistance, and safeguards for control, inhibition, or reversal of drive action.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.