Six years of grazing exclusion is the optimum duration in the alpine meadow-steppe of the north-eastern Qinghai-Tibetan Plateau.

Grazing exclusion is an effective management strategy for restoring degraded grasslands worldwide, but the effects of different exclusion durations on vegetation structure and soil properties remain unclear. Therefore, we evaluated vegetation characteristics and soil properties in an alpine meadow-steppe under grazing exclusion of different lengths (with grazing and with 3-year, 6-year, 9-year and 11-year grazing exclusions) on the Qinghai-Tibetan Plateau (QTP). We also explored the relationships among above-ground biomass, biodiversity and soil properties to ascertain the mechanism underlying the impact of grazing exclusion on these factors. The results showed that the above- and below-ground biomass, total number of plant species, community density, Shannon-Wiener diversity index, evenness index, richness index, soil and vegetation carbon (C) and nitrogen (N) storage and ecosystem C and N storage exhibited a hump-shaped pattern in response to the length of grazing exclusion with a 6-year threshold. In addition, structural equation modelling showed that the bulk density, soil moisture content, micro sand content and clay and silt contents were the most important determining factors leading to an increase in above-ground biomass in the alpine meadow-steppe after grazing exclusion, whereas the soil total N, available N, available phosphate and soil organic C content were the most important determining factors leading to a decrease in biodiversity. Considering the stability of the plant community and the C and N pools, long-term grazing exclusion (>9 years) is unnecessary, and the optimum exclosure duration of the moderately degraded Elymus nutans - Kobresia humilis type alpine meadow-steppe is six years on the north-eastern QTP.