Evidence map›Paper›PMID 30467339›Full record

ArticleScientific reports2018

Effects of Low Intensity Focused Ultrasound on Liposomes Containing Channel proteins.

Meghedi Babakhanian, Limin Yang, Bryan Nowroozi, George Saddik, Lilian Boodaghians, Paul Blount, Warren Grundfest

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 39 citations in OpenAlex.

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  16. Recent developments in imaging of epilepsy.Current opinion in neurology · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Meghedi BabakhanianDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Limin YangDepartment of Physiology, UT Southwestern Medical Center, Dallas, TX, 75390-9040, USA.
Bryan NowrooziDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
George SaddikDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Lilian BoodaghiansDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Paul BlountDepartment of Physiology, UT Southwestern Medical Center, Dallas, TX, 75390-9040, USA. Paul.Blount@utsouthwestern.edu.ORCID http://orcid.org/0000-0001-5259-4881
Warren GrundfestDepartment of Bioengineering, University of California, Los Angeles, CA, 90095, USA. warrenbe@seas.ucla.edu.
University of California, Los Angeles · USSouthwestern Medical CenterSouthwestern Medical Center · US

Funding

UCLA Clinical and Translational Science InstituteUL1TR000124 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2012 to 2015
$57.0M
MOLECULAR MECHANISMS OF MECHANOSENSITIVE CHANNEL GATINGR01GM061028 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI BLOUNT, PAUL · 2000 to 2017
$4.7M
Using Small Compounds as Probes for Studying Mechanosensitive Channel GatingR01GM121780 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI BLOUNT, PAUL · 2017 to 2020
$1.1M
NCATS NIH HHS UL1 TR000124NIGMS NIH HHS R01 GM061028NIGMS NIH HHS R01 GM121780
6 · The paper itself

Abstract

The ability to reversibly and non-invasively modulate region-specific brain activity in vivo suggests Low Intensity Focused Ultrasound (LIFU) as potential therapeutics for neurological dysfunctions such as epilepsy and Parkinson's disease. While in vivo studies provide evidence of the bioeffects of LIFU on neuronal activity, they merely hint at potential mechanisms but do not fully explain how this technology achieves these effects. One potential hypothesis is that LIFU produces local membrane depolarization by mechanically perturbing the neuronal cell membrane, or activating channels or other proteins embedded in the membrane. Proteins that sense mechanical perturbations of the membrane, such as those gated by membrane tension, are prime candidates for activating in response to LIFU and thus leading to the neurological responses that have been measured. Here we use the bacterial mechanosensitive channel MscL, which has been purified and reconstituted in liposomes, to determine how LIFU may affect the activation of this membrane-tension gated channel. Two bacterial voltage-gated channels, KvAP and NaK2K F92A channels were also studied. Surprisingly, the results suggest that ultrasound modulation and membrane perturbation does not induce channel gating, but rather induces pore formation at the membrane protein-lipid interface. However, in vesicles with high MscL mechanosensitive channel concentrations, apparent decreases in pore formation are observed, suggesting that this membrane-tension-sensitive protein may serve to increase the elasticity of the membrane, presumably because of expansion of the channel in the plane of the membrane independent of channel gating.

Indexed as

Cell MembraneIon Channel GatingIon ChannelsLiposomesMechanotransduction, CellularModels, BiologicalUltrasonic WavesIon ChannelsLiposomes

Identifiers

PMID30467339
PMCPMC6250712
OpenAlexW2900998291

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.