Evidence map›Paper›PMID 30466490›Full record

ArticleBMC research notes2018

Improving redox sensitivity of roGFP1 by incorporation of selenocysteine at position 147.

Katherine R Stanford, Joanne M Ajmo, Parmvir K Bahia, Stephen H Hadley, Thomas E Taylor-Clark

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Katherine R StanfordDepartment of Molecular Pharmacology & Physiology, Morsani College of Medicine, University of South Florida, 12901 Bruce B Downs Blvd, Tampa, FL, 33612, USA.
Joanne M AjmoDepartment of Molecular Pharmacology & Physiology, Morsani College of Medicine, University of South Florida, 12901 Bruce B Downs Blvd, Tampa, FL, 33612, USA.
Parmvir K BahiaDepartment of Molecular Pharmacology & Physiology, Morsani College of Medicine, University of South Florida, 12901 Bruce B Downs Blvd, Tampa, FL, 33612, USA.
Stephen H HadleyDepartment of Molecular Pharmacology & Physiology, Morsani College of Medicine, University of South Florida, 12901 Bruce B Downs Blvd, Tampa, FL, 33612, USA.
Thomas E Taylor-ClarkDepartment of Molecular Pharmacology & Physiology, Morsani College of Medicine, University of South Florida, 12901 Bruce B Downs Blvd, Tampa, FL, 33612, USA. ttaylorc@health.usf.edu.ORCID http://orcid.org/0000-0002-5056-2699
University of South Florida · US

Funding

Mitochondrial reactive oxygen species induce airway sensory nerve activityR01HL119802 · NHLBI · UNIVERSITY OF SOUTH FLORIDA · PI TAYLOR-CLARK, THOMAS EDWARD · 2013 to 2017
$2.0M
National Institutes of Health R01HL119802NHLBI NIH HHS R01 HL119802
6 · The paper itself

Abstract

objectiveRedox-sensitive green fluorescent protein (roGFP) is a genetically-encoded redox-sensitive protein used to detect cellular oxidative stress associated with reactive oxygen species production. Here we replaced the cysteine at position 147 of roGFP1 (variant of roGFP) with selenocysteine in order to increase redox sensitivity of the redox reporter.

resultsExpression of roGFP1 selenoprotein (roGFP1-Se147) in HEK293 cells required the presence of a selenocysteine insertion sequence and was augmented by co-expression with SBP2. roGFP1-Se147 demonstrated a similar excitation and emission spectra to roGFP1. Although expression of roGFP1-Se147 was limited, it was sufficient enough to perform live cell imaging to evaluate sensitivity to oxidation and reduction. roGFP1-Se147 exhibited a 100-fold increase in sensitivity to oxidation with H

Indexed as

Antimycin ACysteineElectron TransportGlutathioneGreen Fluorescent ProteinsHEK293 CellsHumansHydrogen PeroxideMitochondriaOxidation-ReductionOxidative StressOxygenReactive Oxygen SpeciesSelenocysteineAntimycin ACysteineGlutathioneGreen Fluorescent ProteinsHydrogen PeroxideOxygenReactive Oxygen SpeciesSelenocysteineGreen fluorescent proteinReactive oxygen speciesRedox-sensingReporterSelenocysteine

Identifiers

PMID30466490
PMCPMC6249920
OpenAlexW2901898137

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.