Evidence map›Paper›PMID 30466455›Full record

ArticleMolecular brain2018

Accelerated FRET-PAINT microscopy.

Jongjin Lee, Sangjun Park, Sungchul Hohng

Abstract read
In one paragraph

Article in Molecular brain, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

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  13. Single-molecule localization microscopy.Nature reviews. Methods primers · 2021
    Article
  14. Review
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jongjin LeeDepartment of Physics and Astronomy, Seoul National University, Seoul, 08826, Republic of Korea.
Sangjun ParkDepartment of Physics and Astronomy, Seoul National University, Seoul, 08826, Republic of Korea.
Sungchul HohngDepartment of Physics and Astronomy, Seoul National University, Seoul, 08826, Republic of Korea. shohng@snu.ac.kr.ORCID 0000-0002-7131-2138

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recent development of FRET-PAINT microscopy significantly improved the imaging speed of DNA-PAINT, the previously reported super-resolution fluorescence microscopy with no photobleaching problem. Here we try to achieve the ultimate speed limit of FRET-PAINT by optimizing the camera speed, dissociation rate of DNA probes, and bleed-through of the donor signal to the acceptor channel, and further increase the imaging speed of FRET-PAINT by 8-fold. Super-resolution imaging of COS-7 microtubules shows that high-quality 40-nm resolution images can be obtained in just tens of seconds.

Indexed as

Fluorescence Resonance Energy TransferMicroscopy, FluorescenceAnimalsBase SequenceChlorocebus aethiopsCOS CellsImage Processing, Computer-AssistedNanotechnologySignal-To-Noise RatioFRETFRET-PAINTSingle-molecule localization microscopySuper-resolution fluorescence microscopy

Identifiers

PMID30466455
PMCPMC6249777

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.