Evidence map›Paper›PMID 30466368›Full record

ArticleReproductive sciences (Thousand Oaks, Calif.)2019

Overexpression of Collapsin Response Mediator Protein 1 Inhibits Human Trophoblast Cells Proliferation, Migration, and Invasion.

Ling Huang, Yuanyuan Li, Chunhui Wang, Na Li, Yue Hou, Ruijing Chang, Man Sun, Ruochen Wang, Lei Zhu, Chong Qiao

Abstract read
PubMed Publisher
In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.4field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Ling Huang *Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, No.36, Sanhao street, Shenyang, Liaoning Province, 110004, China.
Yuanyuan Li *Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, No.36, Sanhao street, Shenyang, Liaoning Province, 110004, China.
Chunhui WangDepartment of Hepatobiliary Surgery, General Hospital of Shenyang Military Region, Shenyang, Liaoning Province, China.
Na LiDepartment of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, No.36, Sanhao street, Shenyang, Liaoning Province, 110004, China.
Yue HouDepartment of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, No.36, Sanhao street, Shenyang, Liaoning Province, 110004, China.
Ruijing ChangDepartment of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
Man SunDepartment of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, No.36, Sanhao street, Shenyang, Liaoning Province, 110004, China.
Ruochen WangDepartment of Biomedical Engineering, University of Wisconsin at Madison, Madison, WI, USA.
Lei ZhuShenyang Academy of Environmental Sciences, Shenyang, Liaoning Province, China.
Chong QiaoDepartment of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, No.36, Sanhao street, Shenyang, Liaoning Province, 110004, China. qiaochong2002@hotmail.com.
China Medical University · CNGeneral Hospital of Shenyang Military Region · CNHebei Medical University · CNShenyang Academy of Environmental Sciences (China) · CNUniversity of Wisconsin–Madison · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Collapsin response mediator protein 1 (CRMP-1) is widely expressed in the nervous system and has tumor suppressive effects. Our previous studies have demonstrated that CRMP-1 was expressed in the trophoblasts of the whole stage of pregnancy with significantly increasing expression in the placenta of early-onset preeclampsia. Preeclampsia, especially early onset, is strongly associated with the dysfunction of trophoblast including proliferation, apoptosis, migration, and invasion. In this study, we found an inhibitory effect of CRMP-1 on proliferation, migration, invasion, and an enhanced effect on apoptosis in human trophoblast cell lines HTR-8/SVneo and JEG-3 by MTT assay, colony formation assay, cell viability assay, caspase 3/7 activity assay, scratch wound assay, and Matrigel Transwell assay. Overexpression of CRMP-1 in trophoblast cells led to downregulate expression of matrix metalloproteinase 2 and 9. The expression of CRMP-1 was detected by real-time quantitative polymerase chain reaction and Western blot analysis. Thus, we suggested that CRMP-1 might have implications for the pathogenesis of preeclampsia by regulating the biological behavior of trophoblast cells.

Indexed as

Cell MovementCell ProliferationApoptosisCaspase 3Caspase 9Cell LineCollapsin Response Mediator Protein 1FemaleHumansMatrix Metalloproteinase 2Matrix Metalloproteinase 9Nerve Tissue ProteinsPre-EclampsiaPregnancyTrophoblastsUp-RegulationCASP3 protein, humanCASP9 protein, humanCaspase 3Caspase 9Collapsin Response Mediator Protein 1CRMP1 protein, humanMatrix Metalloproteinase 2Matrix Metalloproteinase 9MMP2 protein, humanMMP9 protein, humanNerve Tissue Proteinscollapsin response mediator protein 1 (CRMP-1)preeclampsiatrophoblast cells

Identifiers

PMID30466368
OpenAlexW2901501514

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.