ArticleJournal of neuroscience methods2019
Mass synaptometry: High-dimensional multi parametric assay for single synapses.
Article in Journal of neuroscience methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Different durations of cognitive stimulation therapy for Alzheimer's disease: a systematic review and meta-analysis.Clinical interventions in aging · 2019Pooled it
- Review
- Mediterranean vs. Western diet effects on the primate cerebral cortical pre-synaptic proteome: Relationships with the transcriptome and multi-system phenotypes.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2025Article
- mRNA and circRNA mislocalization to synapses are key features of Alzheimer's disease.PLoS genetics · 2024Article
- Cross-species comparative analysis of single presynapses.Scientific reports · 2023Article
- Bringing synapses into focus: Recent advances in synaptic imaging and mass-spectrometry for studying synaptopathy.Frontiers in synaptic neuroscience · 2023Review
- Vesicular Glutamate Release from Feeder-FreehiPSC-Derived Neurons.International journal of molecular sciences · 2022Article
- Array tomography: 15 years of synaptic analysis.Neuronal signaling · 2022Article
- Application of Machine Learning Solutions to Optimize Parameter Prediction to Enhance Automatic NMR Metabolite Profiling.Metabolites · 2022Article
- Cognitive Performance is Associated with Altered Cerebral Hemodynamics Assessed by Transcranial Ultrasound in Parkinson's Disease.Neuropsychiatric disease and treatment · 2022Article
- Towards high throughput and high information coverage: advanced single-cell mass spectrometric techniques.Analytical and bioanalytical chemistry · 2022Review
- Single-synapse analyses of Alzheimer's disease implicate pathologic tau, DJ1, CD47, and ApoE.Science advances · 2021Article
- Mass-tag barcoding for multiplexed analysis of human synaptosomes and other anuclear events.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Article
- Enantiomers of 2-methylglutamate and 2-methylglutamine selectively impact mouse brain metabolism and behavior.Scientific reports · 2021Article
- Article
- Association of Clusterin Levels in Cerebrospinal Fluid with Synaptic Degeneration Across the Alzheimer's Disease Continuum.Neuropsychiatric disease and treatment · 2020Article
- The basis of cellular and regional vulnerability in Alzheimer's disease.Acta neuropathologica · 2019Review
- Long-term icariin treatment ameliorates cognitive deficits via CD4Clinical interventions in aging · 2019Article
- The neuroprotective effect of bisperoxovandium (pyridin-2-squaramide) in intracerebral hemorrhage.Drug design, development and therapy · 2019Article
- Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundSynaptic alterations, especially presynaptic changes, are cardinal features of neurodegenerative diseases and strongly correlate with cognitive decline. NEW
methodWe report "Mass Synaptometry" for the high-dimensional analysis of individual human synaptosomes, enriched nerve terminals from brain. This method was adapted from cytometry by time-of-flight mass spectrometry (CyTOF), which is commonly used for single-cell analysis of immune and blood cells.
resultHere we overcome challenges for single synapse analysis by optimizing synaptosome preparations, generating a 'SynTOF panel,' recalibrating acquisition settings, and applying computational analyses. Through the analysis of 390,000 individual synaptosomes, we also provide proof-of principle validation by characterizing changes in synaptic diversity in Lewy Body Disease (LBD), Alzheimer's disease and normal brain. COMPARISON WITH EXISTING METHOD(S): Current imaging methods to study synapses in humans are capable of analyzing a limited number of synapses, and conventional flow cytometric techniques are typically restricted to fewer than 6 parameters. Our method allows for the simultaneous detection of 34 parameters from tens of thousands of individual synapses.
conclusionWe applied Mass Synaptometry to analyze 34 parameters simultaneously on more than 390,000 synaptosomes from 13 human brain samples. This new approach revealed regional and disease-specific changes in synaptic phenotypes, including validation of this method with the expected changes in the molecular composition of striatal dopaminergic synapses in Lewy body disease and Alzheimer's disease. Mass synaptometry enables highly parallel molecular profiling of individual synaptic terminals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.