Evidence map›Paper›PMID 30465253›Full record

ArticleFood and environmental virology2019

Interlaboratory Validation of a Method for Hepatitis E Virus RNA Detection in Meat and Meat Products.

Nadine Althof, Eva Trojnar, Thomas Böhm, Sabine Burkhardt, Anja Carl, Matthias Contzen, Jochen Kilwinski, Steffen Mergemeier, Dominik Moor, Dietrich Mäde and 1 more

Abstract read
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In one paragraph

Article in Food and environmental virology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Nadine AlthofGerman Federal Institute for Risk Assessment, Berlin, Germany.
Eva TrojnarGerman Federal Institute for Risk Assessment, Berlin, Germany.
Thomas BöhmLandesbetrieb Hessisches Landeslabor, Kassel, Germany.
Sabine BurkhardtLandeslabor Berlin-Brandenburg, Berlin, Germany.
Anja CarlBayrisches Landesamt für Gesundheit und Lebensmittelsicherheit, Erlangen, Germany.
Matthias ContzenChemisches und Veterinäruntersuchungsamt Stuttgart, Fellbach, Germany.
Jochen KilwinskiChemisches und Veterinäruntersuchungsamt Westfalen, Arnsberg, Germany.
Steffen MergemeierCONGEN - Biotechnologie GmbH, Berlin, Germany.
Dominik MoorBundesamt für Lebensmittelsicherheit und Veterinärwesen, Bern, Switzerland.
Dietrich MädeLandesamt für Verbraucherschutz Sachsen-Anhalt, Halle (Saale), Germany.
Reimar JohneGerman Federal Institute for Risk Assessment, Berlin, Germany. Reimar.Johne@bfr.bund.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Increasing numbers of hepatitis E cases are currently recognized in many European countries. The zoonotic hepatitis E virus (HEV) genotype 3 mainly circulates in domestic pigs and wild boars, and can be transmitted to humans via consumption of insufficiently heated meat or meat products produced from those animals. Here, a detailed protocol for detection of HEV RNA in meat products is provided, which is based on the method originally described by Szabo et al. (Intl J Food Microbiol 215:149-156, 2015). It consists of a TRI Reagent®/chloroform-based food matrix homogenization, a silica bead-based RNA extraction and a real-time RT-PCR-based RNA detection. The method was further validated in a ring trial with nine independent laboratories using pork liver sausage samples artificially contaminated with different amounts of HEV. The results indicate sufficient sensitivity, specificity, and accuracy of the method for its broad future use in survey studies, routine food control or outbreak investigations.

Indexed as

RNA, ViralAnimalsHepatitis E virusLimit of DetectionMeatNucleic Acid Amplification TechniquesReproducibility of ResultsSus scrofaVirologyRNA, ViralDetection methodHepatitis E virusMeat productsRing trialValidation

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.