ArticleLab on a chip2018
On-chip oocyte denudation from cumulus-oocyte complexes for assisted reproductive therapy.
Article in Lab on a chip, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 2 syntheses or guidelines pooled it, 54 citations in OpenAlex.
- Microfluidic chips in female reproduction: a systematic review of status, advances, and challenges.Theranostics · 2024Pooled it
- Bioengineering trends in female reproduction: a systematic review.Human reproduction update · 2022Pooled it
- Automation, Artificial Intelligence (AI), and Digital Management in IVF Laboratories: Current Status, Challenges and Potential.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- Advanced three-dimensional culture technologies in reproductive medicine: A narrative review.International journal of reproductive biomedicine · 2026Review
- Microfluidic automation improves oocyte recovery from follicular fluid of patients undergoing in vitro fertilization.Nature medicine · 2026Article
- Microfluidics for in vitro fertilization: from science to clinical validation.Human reproduction update · 2026Review
- Dynamic microdevice culture during bovine oocyte maturation decreases lipid accumulation and improve blastocyst cell numbers.Scientific reports · 2025Article
- Integration of Bioengineered Tools in Assisted Reproductive Technologies.Advanced healthcare materials · 2025Review
- FERTILITY CARE IN LOW- AND MIDDLE- INCOME COUNTRIES: The future use of AI to improve accessibility of assisted reproductive technology in low- and middle-income countries.Reproduction & fertility · 2025Review
- Article
- The Role of Cumulus Cells for The Immature Oocyte Undergoing In Vitro Maturation Rescue: A Retrospective Analysis.International journal of fertility & sterility · 2025Article
- Development of a low-cost microfluidic chip for hyaluronidase-free oocyte denudation in mammals.Biotechnology notes (Amsterdam, Netherlands) · 2025Article
- Single-cell Technology in Stem Cell Research.Current stem cell research & therapy · 2025Review
- A study on the effect of the number of expansion units in a microfluidic chip on hyaluronidase-free oocyte denudation in mammals.Journal of electrical bioimpedance · 2025Article
- Beyond Earth's bounds: navigating the frontiers of Assisted Reproductive Technologies (ART) in space.Reproductive biology and endocrinology : RB&E · 2024Review
- Exploring the potential of in vitro extracellular vesicle generation in reproductive biology.Journal of extracellular biology · 2024Review
- Advances in microfluidic technology for sperm screening and in vitro fertilization.Analytical and bioanalytical chemistry · 2024Review
- Can Microfluidics Improve Sperm Quality? A Prospective Functional Study.Biomedicines · 2024Article
- Design and implementation of a lab-on-a-chip for assisted reproductive technologies.BioImpacts : BI · 2024Article
- Revolutionizing the female reproductive system research using microfluidic chip platform.Journal of nanobiotechnology · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Human infertility can be treated using assisted reproductive technology (ART) such as intracytoplasmic sperm injection (ICSI). But current ART techniques suffer from multiple cumbersome processes requiring technically skilled personnel. Microfluidics technologies offer unique opportunities to streamline ART procedures, reduce stress imposed upon gametes and embryos, and minimize the operator-to-operator variability. However, there have been no automated and continuous processing systems that can reduce the dependence on well-trained embryologists to obtain ICSI-ready oocytes from patients. In this study, using mouse models, we developed a microfluidic device to denude oocytes from the surrounding cumulus-corona cell mass, facilitating the evaluation of oocyte quality and the injection of sperm. Enzyme-treated cumulus-oocyte complexes pass through a series of jagged-surface constriction microchannels of optimized geometries. The jagged inner wall of constriction channels facilitates stripping off of the cumulus-corona cell mass. Oocytes that were denuded by the device showed comparable fertilization and developmental competence compared with mechanical pipetting. The device developed in this study achieves the automation of a manual process for oocyte denudation in a continuous flow, as well as improving standardization and ease-of-use. Our denudation-on-a-chip approach requires inexpensive and simple equipment, which represents one step forward towards improving the accessibility and affordability of assisted reproductive therapy.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.