ArticleNature communications2018
A single Gal4-like transcription factor activates the Crabtree effect in Komagataella phaffii.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 61 citations in OpenAlex.
- An advanced genome engineering platform for Komagataella phaffii enabling versatile in vivo DNA assembly and multiplex integration.Journal of biological engineering · 2026Article
- Convergent evolution of aerobic fermentation through divergent mechanisms acting on key shared glycolytic genes.The EMBO journal · 2026Article
- The return of metabolism: biochemistry and physiology of glycolysis.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Transcriptional regulation: efficient genetic engineering tools for non-conventional yeasts.FEMS yeast research · 2026Review
- Synthetic autotrophic yeast enables high itaconic acid production from COGreen chemistry : an international journal and green chemistry resource : GC · 2025Article
- Convergent evolution of aerobic fermentation through divergent mechanisms acting on key shared glycolytic genes.bioRxiv : the preprint server for biology · 2025Article
- Flo5-1 and Nrg1 are involved in reversible pH-dependent flocculation in Komagataella phaffii.Applied microbiology and biotechnology · 2025Article
- Metabolic Engineering of Komagataella phaffii and Process Optimization for Biosynthesis of 1,2,4-Butanetriol From Xylose.Biotechnology journal · 2025Article
- Impact of Oxygen Availability on the Organelle-Specific Redox Potentials and Stress in Recombinant Protein Producing Komagataella phaffii.Microbial biotechnology · 2025Article
- Metabolic Engineering ofMolecules (Basel, Switzerland) · 2024Article
- Fermenting the future - on the benefits of a bioart collaboration.FEMS yeast research · 2024Article
- Physiological relevance, localization and substrate specificity of the alternative (type II) mitochondrial NADH dehydrogenases ofFrontiers in microbiology · 2024Article
- Advances in Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2023Review
- High throughputMicrobial cell factories · 2023Article
- A Pan-Draft Metabolic Model Reflects Evolutionary Diversity across 332 Yeast Species.Biomolecules · 2022Article
- Advances in Cell Engineering of theMetabolites · 2022Review
- The evolution of the GALactose utilization pathway in budding yeasts.Trends in genetics : TIG · 2022Review
- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering ofFrontiers in bioengineering and biotechnology · 2022Article
- What makes Komagataella phaffii non-conventional?FEMS yeast research · 2021Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Crabtree phenotype defines whether a yeast can perform simultaneous respiration and fermentation under aerobic conditions at high growth rates. It provides Crabtree positive yeasts an evolutionary advantage of consuming glucose faster and producing ethanol to outcompete other microorganisms in sugar rich environments. While a number of genetic events are associated with the emergence of the Crabtree effect, its evolution remains unresolved. Here we show that overexpression of a single Gal4-like transcription factor is sufficient to convert Crabtree-negative Komagataella phaffii (Pichia pastoris) into a Crabtree positive yeast. Upregulation of the glycolytic genes and a significant increase in glucose uptake rate due to the overexpression of the Gal4-like transcription factor leads to an overflow metabolism, triggering both short-term and long-term Crabtree phenotypes. This indicates that a single genetic perturbation leading to overexpression of one gene may have been sufficient as the first molecular event towards respiro-fermentative metabolism in the course of yeast evolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.