Evidence map›Paper›PMID 30463776›Full record

ArticleThe Journal of surgical research2018

Tracking Macrophage Infiltration in a Mouse Model of Pancreatic Cancer with the Positron Emission Tomography Tracer [11C]PBR28.

Mirna Perusina Lanfranca, Jenny Lazarus, Xia Shao, Hari Nathan, Marina Pasca Di Magliano, Weiping Zou, Morand Piert, Timothy L Frankel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of surgical research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.8field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 22 citations in OpenAlex.

  1. Preclinical Fluorescence-Guided Imaging Leveraging Surrounding Sentinel Tumor Microenvironment Identifies High-Risk Premalignant Pancreatic Lesions.Clinical cancer research : an official journal of the American Association for Cancer Research · 2025
    Article
  2. Review
  3. Review
  4. Peptide-Based [Pharmaceutics · 2022
    Article
  5. Review
  6. Review
  7. Review
  8. Article
  9. Polymer nanoparticle-assisted chemotherapy of pancreatic cancer.Therapeutic advances in medical oncology · 2020
    Review
  10. Cell Tracking in Cancer Immunotherapy.Frontiers in medicine · 2020
    Review
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Mirna Perusina LanfrancaDepartment of Surgery, University of Michigan, Ann Arbor, Michigan.
Jenny LazarusDepartment of Surgery, University of Michigan, Ann Arbor, Michigan.
Xia ShaoDepartment of Radiology, University of Michigan, Ann Arbor, Michigan.
Hari NathanDepartment of Surgery, University of Michigan, Ann Arbor, Michigan.
Marina Pasca Di MaglianoDepartment of Surgery, University of Michigan, Ann Arbor, Michigan.
Weiping ZouDepartment of Surgery, University of Michigan, Ann Arbor, Michigan.
Morand PiertDepartment of Radiology, University of Michigan, Ann Arbor, Michigan.
Timothy L FrankelDepartment of Surgery, University of Michigan, Ann Arbor, Michigan. Electronic address: timofran@med.umich.edu.
University of Michigan–Ann Arbor · US

Funding

Role of Interleukin-22 and Innate Lymphoid Cells in Pancreas Cancer Initiation and ProgressionK08CA201581 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FRANKEL, TIMOTHY LOUIS · 2016 to 2020
$703k
NCI NIH HHS K08 CA201581
6 · The paper itself

Abstract

backgroundThe tumor microenvironment of pancreatic ductal adenocarcinoma (PDAC) contains abundant immunosuppressive tumor-associated macrophages. High level of infiltration is associated with poor outcome and is thought to represent a major roadblock to lymphocyte-based immunotherapy. Efforts to block macrophage infiltration have been met with some success, but noninvasive means to track tumor-associated macrophagess in PDAC are lacking. Translocator protein (TSPO) is a mitochondrial membrane receptor which is upregulated in activated macrophages. We sought to identify if a radiotracer-labeled cognate ligand could track macrophages in PDAC. MATERIALS AND

methodsA murine PDAC cell line was established from a transgenic mouse with pancreas-specific mutations in KRAS and p53. After confirming lack of endogenous TSPO expression, tumors were established in syngeneic mice. A radiolabeled TSPO-specific ligand ([11C] peripheral benzodiazepine receptor [PBR]28) was delivered intravenously, and tumor uptake was assessed by autoradiography, ex vivo, or micro-positron emission tomography imaging.

resultsResected tumors contained abundant macrophages as determined by immunohistochemistry and flow cytometry. Immunoblotting revealed murine macrophages expressed TSPO with increasing concentration on activation and polarization. Autoradiography of resected tumors confirmed [11C]PBR28 uptake, and whole mount sections demonstrated the ability to localize tumors. To confirm the findings were macrophage specific, experiments were repeated in CD11b-deficient mice, and the radiotracer uptake was diminished. Micro-positron emission tomography imaging validated radiotracer uptake and tumor localization in a clinically applicable manner.

conclusionsAs new immunotherapeutics reshape the PDAC microenvironment, tools are needed to better measure and track immune cell subsets. We have demonstrated the potential to measure changes in macrophage infiltration in PDAC using [11C]PBR28.

Indexed as

Carbon RadioisotopesAcetamidesAnimalsCarcinoma, Pancreatic DuctalCell Line, TumorDisease Models, AnimalFemaleMacrophagesMaleMiceMice, TransgenicPancreatic NeoplasmsPositron-Emission TomographyPyridinesReceptors, GABATumor MicroenvironmentAcetamidesBzrp protein, mouseCarbon-11Carbon RadioisotopesN-(2-methoxybenzyl)-N-(4-phenoxypyridin-3-yl)acetamidePyridinesReceptors, GABAPancreatic cancerPET imagingTumor associated macrophages

Identifiers

PMID30463776
PMCPMC6251503
OpenAlexW2886031364

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.