ArticleJournal of ovarian research2018
Is the aging human ovary still ticking?: Expression of clock-genes in luteinized granulosa cells of young and older women.
Article in Journal of ovarian research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 27 citations in OpenAlex.
- Review
- Shift Work as a Potential Risk Factor for Lower Ovarian Reserve: A Study of Fertility Patients.Journal of clinical medicine · 2026Article
- Ovarian Rev-erbα: a central regulator of fertility following chronodisruption.Frontiers in endocrinology · 2026Article
- A Multi-Ingredient Supplement Improves Body Re-Composition, Ovarian Aging Markers, and Reproductive Success in Young and Middle-Aged Female Mice.Biomolecules · 2025Article
- Twelve-hour ultradian rhythmic reprogramming of gene expression in the human ovary during aging.Journal of assisted reproduction and genetics · 2025Article
- Effects of Age and MPTP-Induced Parkinson's Disease on the Expression of Genes Associated with the Regulation of the Sleep-Wake Cycle in Mice.International journal of molecular sciences · 2024Article
- Mitigating Age-Related Ovarian Dysfunction with the Anti-Inflammatory Agent MIT-001.International journal of molecular sciences · 2023Article
- CLOCK inhibits the proliferation of porcine ovarian granulosa cells by targeting ASB9.Journal of animal science and biotechnology · 2023Article
- Relationship between cognitive dysfunction and the promoter methylation of PER1 and CRY1 in patients with cerebral small vessel disease.Frontiers in aging neuroscience · 2023Article
- Melatonin, Clock Genes, and Mammalian Reproduction: What Is the Link?International journal of molecular sciences · 2021Review
- Circadian Rhythms Within the Female HPG Axis: From Physiology to Etiology.Endocrinology · 2021Review
- Disruption of Circadian Rhythms: A Crucial Factor in the Etiology of Infertility.International journal of molecular sciences · 2020Review
- Age-Induced Differential Changes in the Central and Colonic Human Circadian Oscillators.International journal of molecular sciences · 2020Article
- Selection of suitable reference genes for core clock gene expression analysis by real-time qPCR in rat ovary granulosa cells.Molecular biology reports · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIt has been shown - mostly in animal models - that circadian clock genes are expressed in granulosa cells and in corpora luteum and might be essential for the ovulatory process and steroidogenesis.
objectiveWe sought to investigate which circadian clock genes exist in human granulosa cells and whether their expression and activity decrease during aging of the ovary. STUDY
designHuman luteinized granulosa cells were isolated from young (age 18-33) and older (age 39-45) patients who underwent in-vitro fertilization treatment. Levels of clock genes expression were measured in these cells 36 h after human chorionic gonadotropin stimulation.
methodsHuman luteinized granulosa cells were isolated from follicular fluid during oocyte retrieval. The mRNA expression levels of the circadian genes CRY1, CRY2, PER1, PER2, CLOCK, ARNTL, ARNTL2, and NPAS2 were analyzed by quantitative polymerase chain reaction.
resultsWe found that the circadian genes CRY1, CRY2, PER1, PER2, CLOCK, ARNTL, ARNTL2, and NPAS2, are expressed in cultured human luteinized granulosa cells. Among these genes, there was a general trend of decreased expression in cells from older women but it reached statistical significance only for PER1 and CLOCK genes (fold change of 0.27 ± 0.14; p = 0.03 and 0.29 ± 0.16; p = 0.05, respectively).
conclusionsThis preliminary report indicates that molecular circadian clock genes exist in human luteinized granulosa cells. There is a decreased expression of some of these genes in older women. This decline may partially explain the decreased fertility and steroidogenesis of reproductive aging.
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