Evidence map›Paper›PMID 30463338›Full record

ArticleSensors (Basel, Switzerland)2018

Nanobody-Alkaline Phosphatase Fusion Protein-Based Enzyme-Linked Immunosorbent Assay for One-Step Detection of Ochratoxin A in Rice.

Zhichang Sun, Xuerou Wang, Qi Chen, Yonghuan Yun, Zongwen Tang, Xing Liu

Abstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
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  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Zhichang SunCollege of Food Science and Technology, Hainan University, 58 Renmin Avenue, Haikou 570228, China. 13919003678@163.com.
Xuerou WangCollege of Food Science and Technology, Hainan University, 58 Renmin Avenue, Haikou 570228, China. w490487@126.com.
Qi ChenCollege of Food Science and Technology, Hainan University, 58 Renmin Avenue, Haikou 570228, China. qichen@hainu.edu.cn.
Yonghuan YunCollege of Food Science and Technology, Hainan University, 58 Renmin Avenue, Haikou 570228, China. yunyonghuan@foxmail.com.
Zongwen TangCollege of Food Science and Technology, Hainan University, 58 Renmin Avenue, Haikou 570228, China. tzwcx@163.com.
Xing LiuCollege of Food Science and Technology, Hainan University, 58 Renmin Avenue, Haikou 570228, China. xliu@hainu.edu.cn.ORCID 0000-0002-1301-4837

Funding

Hainan University kyqd1631National Natural Science Foundation of China 31760493
6 · The paper itself

Abstract

Ochratoxin A (OTA) has become one a focus of public concern because of its multiple toxic effects and widespread contamination. To monitor OTA in rice, a sensitive, selective, and one-step enzyme-linked immunosorbent assay (ELISA) using a nanobody-alkaline phosphatase fusion protein (Nb28-AP) was developed. The Nb28-AP was produced by auto-induction expression and retained an intact antigen-binding capacity and enzymatic activity. It exhibited high thermal stability and organic solvent tolerance. Under the optimal conditions, the developed assay for OTA could be finished in 20 min with a half maximal inhibitory concentration of 0.57 ng mL

Indexed as

Alkaline PhosphataseCross ReactionsEnzyme-Linked Immunosorbent AssayHydrogen-Ion ConcentrationLimit of DetectionOchratoxinsOryzaRecombinant Fusion ProteinsSingle-Domain AntibodiesAlkaline Phosphataseochratoxin Aochratoxin Bochratoxin COchratoxinsRecombinant Fusion ProteinsSingle-Domain Antibodiesalkaline phosphataseimmunoassaymycotoxinnanobody

Identifiers

PMID30463338
PMCPMC6263964

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.