ArticleNucleic acids research2019
Stem-loops direct precise processing of 3' UTR-derived small RNA MicL.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Identification of proteins interacting with the N-terminal half of endoribonuclease RNase E inRNA biology · 2026Article
- What makes a small RNA work?Nucleic acids research · 2025Review
- Cooperation of regulatory RNA and the RNA degradosome in transcript surveillance.Nucleic acids research · 2024Article
- Function and mechanism of action of the small regulatory RNA ArcZ inRNA (New York, N.Y.) · 2024Review
- Role of the 5' end phosphorylation state for small RNA stability and target RNA regulation in bacteria.Nucleic acids research · 2023Article
- Relaxed Cleavage Specificity of Hyperactive Variants of Escherichia coli RNase E on RNA I.Journal of microbiology (Seoul, Korea) · 2023Article
- Structure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis.The EMBO journal · 2023Article
- The recognition of structured elements by a conserved groove distant from domains associated with catalysis is an essential determinant of RNase E.Nucleic acids research · 2023Article
- The stationary phase-specific sRNA FimR2 is a multifunctional regulator of bacterial motility, biofilm formation and virulence.Nucleic acids research · 2022Article
- A 3' UTR-derived small RNA connecting nitrogen and carbon metabolism in enteric bacteria.Nucleic acids research · 2022Article
- An overview of gene regulation in bacteria by small RNAs derived from mRNA 3' ends.FEMS microbiology reviews · 2022Review
- Impact of pseudouridylation, substrate fold, and degradosome organization on the endonuclease activity of RNase E.RNA (New York, N.Y.) · 2021Article
- Adaptor protein RapZ activates endoribonuclease RNase E by protein-protein interaction to cleave a small regulatory RNA.RNA (New York, N.Y.) · 2020Article
- Article
- Prevalence of small base-pairing RNAs derived from diverse genomic loci.Biochimica et biophysica acta. Gene regulatory mechanisms · 2020Review
- Switching fatty acid metabolism by an RNA-controlled feed forward loop.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Differential evolution in 3'UTRs leads to specific gene expression in Staphylococcus.Nucleic acids research · 2020Article
- Feedback regulation of small RNA processing by the cleavage product.RNA biology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Increasing numbers of 3'UTR-derived small, regulatory RNAs (sRNAs) are being discovered in bacteria, most generated by cleavage from longer transcripts. The enzyme required for these cleavages has been reported to be RNase E, the major endoribonuclease in enterica bacteria. Previous studies investigating RNase E have come to a range of different conclusions regarding the determinants for RNase E processing. To better understand the sequence and structure determinants for the precise processing of a 3' UTR-derived sRNA, we examined the cleavage of multiple mutant and chimeric derivatives of the 3' UTR-derived MicL sRNA in vivo and in vitro. Our results revealed that tandem stem-loops 3' to the cleavage site define optimal, correctly-positioned cleavage of MicL and probably other sRNAs. Moreover, our assays of MicL, ArcZ and CpxQ showed that sRNAs exhibit differential sensitivity to RNase E, likely a consequence of a hierarchy of sRNA features recognized by the endonuclease.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.