ArticleMicrobial biotechnology2020
Engineering Pseudomonas protegens Pf-5 to improve its antifungal activity and nitrogen fixation.
Article in Microbial biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Horizon scanning of potential environmental applications of terrestrial animals, fish, algae and microorganisms produced by genetic modification, including the use of new genomic techniques.Frontiers in genome editing · 2024Pooled it
- TheApplied and environmental microbiology · 2026Article
- Root-zone waterproof cultivation advanced coloring by accumulation anthocyanins and modulated epiphytic bacteria rebuilding in Marselan grape.BMC microbiology · 2026Article
- Mechanisms of Pseudomonas spp. as plant growth promoting rhizobacteria: plant nutrition and biocontrol.Antonie van Leeuwenhoek · 2026Review
- Engineering Pseudomonas protegens as a Targeted Antifungal-Effector Delivery Chassis via Heterologous Type III Secretion System Reconstitution.Microbial biotechnology · 2026Article
- Dynamics of genetic circuits in Pseudomonas protegens.Cell systems · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Pseudomonas protegens as a biocontrol agent against phytopathogenic fungi - mini review.World journal of microbiology & biotechnology · 2025Review
- Agricultural biotechnology in China: product development, commercialization, and perspectives.aBIOTECH · 2025Review
- Effect ofLife (Basel, Switzerland) · 2025Article
- Plant-microbe interactions: PGPM as microbial inoculants/biofertilizers for sustaining crop productivity and soil fertility.Current research in microbial sciences · 2025Review
- AFrontiers in systems biology · 2025Article
- A Homolog of the Histidine Kinase RetS Controls the Synthesis of Alginates, PHB, Alkylresorcinols, and Motility in Azotobacter vinelandii.Current microbiology · 2024Article
- New Insights intoAntibiotics (Basel, Switzerland) · 2024Review
- Genome Analysis ofJournal of fungi (Basel, Switzerland) · 2024Article
- Precise genome engineering in Pseudomonas using phage-encoded homologous recombination and the Cascade-Cas3 system.Nature protocols · 2023Review
- The emerging role of recombineering in microbiology.Engineering microbiology · 2023Review
- Microbial biotechnology in the effort to end hunger.Microbial biotechnology · 2023Article
- Technological Quality of Sugarcane Inoculated with Plant-Growth-Promoting Bacteria and Residual Effect of Phosphorus Rates.Plants (Basel, Switzerland) · 2023Article
- Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity.Microbial cell factories · 2023Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In agricultural production, sustainability is currently one of the most significant concerns. The genetic modification of plant growth-promoting rhizobacteria may provide a novel way to use natural bacteria as microbial inoculants. In this study, the root-colonizing strain Pseudomonas protegens Pf-5 was genetically modified to act as a biocontrol agent and biofertilizer with biological nitrogen fixation activity. Genetic inactivation of retS enhanced the production of 2,4-diacetylphloroglucinol, which contributed for the enhanced antifungal activity. Then, the entire nitrogenase island with native promoter from Pseudomonas stutzeri DSM4166 was introduced into a retS mutant strain for expression. Root colonization patterns assessed via confocal laser scanning microscopy confirmed that GFP-tagged bacterial were mainly located on root surfaces and at the junctions between epidermal root cells. Moreover, under pathogen and N-limited double treatment conditions, the fresh weights of seedlings inoculated with the recombinant retS mutant-nif strain were increased compared with those of the control. In conclusion, this study has innovatively developed an eco-friendly alternative to the agrochemicals that will benefit global plant production significantly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.