Evidence map›Paper›PMID 30460651›Full record

ArticleEnvironmental science and pollution research international2019

The size-dependent genotoxicity and oxidative stress of silica nanoparticles on endothelial cells.

Furong Zhou, Fen Liao, Lingying Chen, Yuanfeng Liu, Wuxiang Wang, Shaolong Feng

Abstract read
PubMed Publisher
In one paragraph

Article in Environmental science and pollution research international, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Review
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Furong ZhouThe School of Public Health, University of South China, Hengyang, 421001, China.
Fen LiaoThe School of Public Health, University of South China, Hengyang, 421001, China.
Lingying ChenThe First Affiliated Hospital, University of South China, Hengyang, 421001, China.
Yuanfeng LiuThe School of Public Health, University of South China, Hengyang, 421001, China.
Wuxiang WangThe School of Public Health, University of South China, Hengyang, 421001, China.
Shaolong FengThe School of Public Health, University of South China, Hengyang, 421001, China. slfeng71@usc.edu.cn.ORCID http://orcid.org/0000-0002-6757-8275

Funding

The Foundation of University of South China 2012XQD44The Science and Technology Foundation of Guangdong Province 2014B030301060
6 · The paper itself

Abstract

Concerns over the health risk of the widely distributed, commonly used silica nanoparticles (SiNPs) are increasing worldwide. Yet, up to now, there are still many major knowledge gaps over the potential adverse effects of SiNP exposure on human cardiovascular health and the underlying mechanisms. In this study, comet assay and micronucleus test were employed to determine the genotoxic potentials of four sizes (10, 25, 50, 100 nm) of SiNPs to human umbilical vein endothelial cells (HUVECs) in culture. The intracellular redox statuses were explored through the determination of the levels of reactive oxygen species (ROS) and reduced glutathione (GSH) with kits, respectively. The protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2) were also detected by Western blot. The results showed that at the administrative levels (1, 5, 25 μg/mL), all the four sizes of SiNPs could induce an increase of both DNA damages and MN frequencies in HUVECs in culture, with a positive dose- and negative size-dependent effect relationship (S100 < S50 < S25 < S10). Also, significantly enhanced levels of intracellular ROS, but decreased levels of GSH, were observed in the SiNP-treated groups. Interestingly, a very similar manner of dose- and size-dependent effect relationship was observed between the ROS test and both comet assay and MN test, but contrary to that of GSH assay. Correspondingly, the levels of Nrf2 protein were also enhanced in the SiNP-treated HUVECs, with a negative size-dependent effect relationship. These results implicated that induction of oxidative stress and subsequent genotoxicity may be an important biological mechanism by which SiNP exposure may affect human cardiovascular health.

Indexed as

Toxicity TestsDNA DamageEndothelial CellsHumansNanoparticlesOxidative StressReactive Oxygen SpeciesSilicon DioxideReactive Oxygen SpeciesSilicon DioxideEndothelial cell (EC)GenotoxicityOxidative stressSilica nanoparticle (SiNP)

Identifiers

PMID30460651

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.