ArticleMicrobial ecology2019
Delivery of a Genetically Marked Serratia AS1 to Medically Important Arthropods for Use in RNAi and Paratransgenic Control Strategies.
Article in Microbial ecology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Serratia marcescens and Enterobacter cloacae exhibit differential population dynamics in Anopheles gambiae s.l after sugar and blood feeding.Malaria journal · 2026Article
- Environmental persistence and transmission dynamics ofApplied and environmental microbiology · 2025Article
- Review
- Harnessing engineered symbionts to combat concurrent malaria and arboviruses transmission.Nature communications · 2025Article
- Isolation and characterization of the midgut microbiota of Aedes albopictus to identify suitable candidates for paratransgenesis.European journal of public health · 2025Article
- Interventions for the control of Crimean-Congo hemorrhagic fever and tick vectors.NPJ vaccines · 2024Review
- Comparative analysis of the microbiota of sand fly vectors of Leishmania major and L. tropica in a mixed focus of cutaneous leishmaniasis in southeast Tunisia; ecotype shapes the bacterial community structure.PLoS neglected tropical diseases · 2024Article
- Assessing Survival of Transgenic Bacteria,Journal of arthropod-borne diseases · 2024Article
- Susceptibility ofJournal of arthropod-borne diseases · 2023Article
- Rodents as vehicle for delivery of transgenic bacteria to make paratransgenic sand fly vectors of cutaneous leishmaniasis in field condition.Scientific reports · 2023Article
- Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector.iScience · 2023Article
- The Impact of RNA Interference in Tick Research.Pathogens (Basel, Switzerland) · 2022Article
- Comparative analysis of the gut microbiota of sand fly vectors of zoonotic visceral leishmaniasis (ZVL) in Iran; host-environment interplay shapes diversity.PLoS neglected tropical diseases · 2022Article
- A Genetically Modified Anti-Plasmodium Bacterium Is Harmless to the Foragers of the Stingless Bee Partamona helleri.Microbial ecology · 2022Article
- Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors.Parasites & vectors · 2022Review
- Evaluation of anti-malaria potency of wild and genetically modified Enterobacter cloacae expressing effector proteins in Anopheles stephensi.Parasites & vectors · 2022Article
- An integrated overview of the bacterial flora composition of Hyalomma anatolicum, the main vector of CCHF.PLoS neglected tropical diseases · 2021Article
- Culturable bacteria associated with Anopheles darlingi and their paratransgenesis potential.Malaria journal · 2021Article
- Proposed Integrated Control of ZoonoticTropical medicine and infectious disease · 2020Review
- Altered Gut Microbiota and Immunity DefinesFrontiers in immunology · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Understanding how arthropod vectors acquire their bacteria is essential for implementation of paratransgenic and RNAi strategies using genetically modified bacteria to control vector-borne diseases. In this study, a genetically marked Serratia AS1 strain expressing the mCherry fluorescent protein (mCherry-Serratia) was used to test various acquisition routes in six arthropod vectors including Anopheles stephensi, Culex pipiens, Cx. quinquefaciatus, Cx. theileri, Phlebotomus papatasi, and Hyalomma dromedarii. Depending on the species, the bacteria were delivered to (i) mosquito larval breeding water, (ii) host skin, (iii) sugar bait, and (iv) males (paratransgenic). The arthropods were screened for the bacteria in their guts or other tissues. All the hematophagous arthropods were able to take the bacteria from the skin of their hosts while taking blood meal. The mosquitoes were able to take up the bacteria from the water at larval stages and to transfer them transstadially to adults and finally to transfer them to the water they laid eggs in. The mosquitoes were also able to acquire the bacteria from male sperm. The level of bacterial acquisition was influenced by blood feeding time and strategies (pool or vessel feeding), dipping in water and resting time of newly emerged adult mosquitoes, and the disseminated tissue/organ. Transstadial, vertical, and venereal bacterial acquisition would increase the sustainability of the modified bacteria in vector populations and decrease the need for supplementary release experiments whereas release of paratransgenic males that do not bite has fewer ethical issues. Furthermore, this study is required to determine if the modified bacteria can be introduced to arthropods in the same routes in nature.
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