ArticleBiomedical optics express2018
Slide-free virtual histochemistry (Part I): development via nonlinear optics.
Article in Biomedical optics express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Label-free multimodal nonlinear microscopy enabled by an optical parametric generator.APL photonics · 2026Article
- Artificial Intelligence in Label-Free Optical Imaging Applications.Methods in molecular biology (Clifton, N.J.) · 2026Article
- On the importance of simultaneous label-free multimodal nonlinear optical imaging for biomedical applications.APL photonics · 2025Article
- Accelerating biopharmaceutical cell line selection with label-free multimodal nonlinear optical microscopy and machine learning.Communications biology · 2025Article
- Inspiring a convergent engineering approach to measure and model the tissue microenvironment.Heliyon · 2024Review
- Analog multiplexing of a laser clock and computational photon counting for fast fluorescence lifetime imaging microscopy.Biomedical optics express · 2024Article
- Toward next-generation endoscopes integrating biomimetic video systems, nonlinear optical microscopy, and deep learning.Biophysics reviews · 2023Review
- Weakly supervised identification of microscopic human breast cancer-related optical signatures from normal-appearing breast tissue.Biomedical optics express · 2023Article
- Tracking the binding of multi-functional fluorescent tags for Alzheimer's disease using quantitative multiphoton microscopy.Journal of biophotonics · 2022Article
- Deep learning augmented microscopy: a faster, wider view, higher resolution autofluorescence-harmonic microscopy.Light, science & applications · 2022Article
- CoreView: fresh tissue biopsy assessment at the bedside using a millifluidic imaging chip.Lab on a chip · 2022Article
- Label-free metabolic and structural profiling of dynamic biological samples using multimodal optical microscopy with sensorless adaptive optics.Scientific reports · 2022Article
- Multimodal microscopy for the simultaneous visualization of five different imaging modalities using a single light source.Biomedical optics express · 2021Article
- Label-Free Deep Profiling of the Tumor Microenvironment.Cancer research · 2021Article
- Label-Free Multimodal Multiphoton Intravital Imaging.Advances in experimental medicine and biology · 2021Article
- Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives.BME frontiers · 2021Review
- Real-time three-dimensional histology-like imaging by label-free nonlinear optical microscopy.Quantitative imaging in medicine and surgery · 2020Article
- Simultaneous two-photon activation and imaging of neural activity based on spectral-temporal modulation of supercontinuum light.Neurophotonics · 2020Article
- Label-free visualization and characterization of extracellular vesicles in breast cancer.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Simultaneous label-free autofluorescence-multiharmonic microscopy and beyond.APL photonics · 2019Article
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Authors and funding
7 authors.
Funding
Abstract
Histochemistry is a microscopy-based technology widely used to visualize the molecular distribution in biological tissue. Recent developments in label-free optical imaging has demonstrated the potential to replace the conventional histochemical labels/markers (fluorescent antibodies, organic dyes, nucleic acid probes, and other contrast agents) with diverse optical interactions to generate histochemical contrasts, allowing "virtual" histochemistry in three spatial dimensions without preparing a microscope slide (i.e. labor-intensive sample preparation). However, the histochemical information in a label-free optical image has often been rather limited due to the difficulty in simultaneously generating multiple histochemical contrasts with strict spatial co-registration. Here, in the first part (Part I) of this two-part series study, we develop a technique of slide-free virtual histochemistry based on label-free multimodal multiphoton microscopy, and simultaneously generate up to four histochemical contrasts from
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.