Evidence map›Paper›PMID 30460113›Full record

ArticleAnimal cells and systems2018

The effects of mouse ovarian granulosa cell function and related gene expression by suppressing BMP/Smad signaling pathway.

Li Zhang, Hejian Wang, Daolun Yu, Jie Chen, Chaofeng Xing, Jie Li, Jun Li, Yafei Cai

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In one paragraph

Article in Animal cells and systems, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Expression pattern ofJournal of advanced veterinary and animal research · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Li ZhangCollege of Life Science, Anhui provincial Key Lab of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu, People's Republic of China.
Hejian WangCollege of Life Science, Anhui provincial Key Lab of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu, People's Republic of China.
Daolun YuCollege of Life Science, Anhui provincial Key Lab of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu, People's Republic of China.
Jie ChenCollege of Life Science, Anhui provincial Key Lab of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu, People's Republic of China.
Chaofeng XingCollege of Life Science, Anhui provincial Key Lab of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu, People's Republic of China.
Jie LiCollege of Life Science, Anhui provincial Key Lab of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu, People's Republic of China.
Jun LiCollege of Life Science, Anhui provincial Key Lab of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu, People's Republic of China.
Yafei CaiCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, People's Republic of China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BMP I type receptor inhibitor can selectively inhibit BMP/Smad signaling pathways, mainly by inhibiting the BMP I type receptor activity to prevent phosphorylation of Smad1, Smad5 and Smad9. The aim of the present study was to explore the effects of mouse ovarian granulosa cell function and related gene expression by suppressing BMP/Smad signaling pathway with LDN-193189(A type of BMP I type receptor inhibitor). In this study, we cultivate the original generation of mouse ovarian granular cells then collect cells and cell culture medium after treatment. Cellular localization and expression of Smad9 and P-smad9 proteins was studied by immunofluorescence (IF) in the ovarian granulosa cells of mouse; Related genes mRNA and proteins expression was checked by QRT-PCR and Western blot; Detected the concentration of related hormones by using ELISA kit; finally, the growth of the cells was analyzed by plotting cell growth curve with CCK-8 assay. The results indicate that, suppression of BMP/Smad signaling pathway can inhibit the expression of LHR and FSHR, inhibit cell proliferation and decrease E

Indexed as

BMP-4BMP/Smad signal pathwayGranulosa cellsSmad9

Identifiers

PMID30460113
PMCPMC6171428

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.