ArticleScientific reports2018
Stable pH Suppresses Defense Signaling and is the Key to Enhance Agrobacterium-Mediated Transient Expression in Arabidopsis Seedlings.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- An Efficient Regeneration and Transient Transformation System for the Desert HalophytePlants (Basel, Switzerland) · 2026Article
- Comprehensive Optimization of Agroinfiltration in Transient Expression System in Nicotiana tabacum with RUBY Visible Reporter.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections · 2026Article
- Floral stage optimization and immune evasion enhance Agrobacterium-mediated genome editing in Arabidopsis.The New phytologist · 2026Article
- Production and purification of tag-free recombinant human acid sphingomyelinase in Nicotiana benthamiana.Plant cell reports · 2025Article
- A review of factors affecting the success of geminivirus infectious clones.Plant cell reports · 2025Review
- An optimized transformation protocol for Anthoceros agrestis and three more hornwort species.The Plant journal : for cell and molecular biology · 2023Article
- A SNARE-like protein fromFrontiers in plant science · 2023Article
- Relative Protein Lifetime Measurement in Plants Using Tandem Fluorescent Protein Timers.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation.Nature communications · 2022Article
- Two interacting transcriptional coactivators cooperatively control plant immune responses.Science advances · 2021Article
- A Highly EfficientPlant communications · 2020Article
- Transient Gene Expression is an Effective Experimental Tool for the Research into the Fine Mechanisms of Plant Gene Function: Advantages, Limitations, and Solutions.Plants (Basel, Switzerland) · 2020Review
- Cucumber mosaic virus-induced gene silencing in banana.Scientific reports · 2019Article
- pH effects on plant calcium fluxes: lessons from acidification-mediated calcium elevation induced by the γ-glutamyl-leucine dipeptide identified from Phytophthora infestans.Scientific reports · 2019Article
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Authors and funding
5 authors.
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Abstract
Agrobacterium-mediated transient expression is a powerful analysis platform for diverse plant gene functional studies, but the mechanisms regulating the expression or transformation levels are poorly studied. Previously, we developed a highly efficient and robust Agrobacterium-mediated transient expression system, named AGROBEST, for Arabidopsis seedlings. In this study, we found that AGROBEST could promote the growth of agrobacteria as well as inhibit the host immunity response. When the factor of agrobacterial growth is minimized, maintaining pH at 5.5 with MES buffer was the key to achieving optimal transient expression efficiency. The expression of plant immunity marker genes, FRK1 and NHL10, was suppressed in the pH-buffered medium as compared with non-buffered conditions in Col-0 and an efr-1 mutant lacking the immunity receptor EFR recognizing EF-Tu, a potent pathogen- or microbe-associated molecular pattern (PAMP or MAMP) of A. tumefaciens. Notably, such immune suppression could also occur in Arabidopsis seedlings without Agrobacterium infection. Furthermore, the PAMP-triggered influx of calcium ions was compromised in the pH-buffered medium. We propose that the enhanced transient expression efficiency by stable pH was due to inhibiting calcium ion uptake and subsequently led to suppressing immunity against Agrobacterium.
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