ArticleProceedings of the National Academy of Sciences of the United States of America2018
Intrinsic cellular chirality regulates left-right symmetry breaking during cardiac looping.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 74 citations in OpenAlex.
- Curvature-Directed Elongation and Chiral Tilting Drive Asymmetric Tissue Patterning.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Actin based cell chirality emerging at the boundary of 2D-microtissue directs chiral multicellular pattern formation.Nature communications · 2026Article
- Contractile forces direct the chiral swirling of minimal cell collectives.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Ventricular Topology in Congenital Heart Defects Associated with Heterotaxy: Can We Find Patterns Reflecting the Syndrome-Specific Tendency for Visceral Symmetry?Journal of cardiovascular development and disease · 2025Article
- Article
- Epithelial cell chirality emerges through the dynamic concentric pattern of actomyosin cytoskeleton.eLife · 2025Article
- morphoHeart: A quantitative tool for integrated 3D morphometric analyses of heart and ECM during embryonic development.PLoS biology · 2025Article
- Energetic scaling behavior of patterned epithelium.Journal of biomechanics · 2024Article
- Dynamics of multicellular swirling on micropatterned substrates.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Biomechanical Modeling of Cell Chirality and Symmetry Breaking of Biological Systems.Mechanobiology in medicine · 2024Article
- Asymmetrical positioning of cell organelles reflects the cell chirality of mouse myoblast cells.APL bioengineering · 2024Article
- Helical vasculogenesis driven by cell chirality.Science advances · 2024Article
- Molecular Pathways and Animal Models of Defects in Situs.Advances in experimental medicine and biology · 2024Article
- Establishment of Cardiac Laterality.Advances in experimental medicine and biology · 2024Review
- The Actin Crosslinker Fascin Regulates Cell Chirality.Advanced biology · 2023Article
- Human Heart Morphogenesis: A New Vision Based on In Vivo Labeling and Cell Tracking.Life (Basel, Switzerland) · 2023Review
- Cell jamming regulates epithelial chiral morphogenesis.Journal of biomechanics · 2023Article
- Genome Editing and Myocardial Development.Advances in experimental medicine and biology · 2023Review
- Direct observation of long-range chirality transfer in a self-assembled supramolecular monolayer at interface in situ.Nature communications · 2022Article
- Interacting with tumor cells weakens the intrinsic clockwise chirality of endothelial cells.APL bioengineering · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The vertebrate body plan is overall symmetrical but left-right (LR) asymmetric in the shape and positioning of internal organs. Although several theories have been proposed, the biophysical mechanisms underlying LR asymmetry are still unclear, especially the role of cell chirality, the LR asymmetry at the cellular level, on organ asymmetry. Here with developing chicken embryos, we examine whether intrinsic cell chirality or handedness regulates cardiac C looping. Using a recently established biomaterial-based 3D culture platform, we demonstrate that chick cardiac cells before and during C looping are intrinsically chiral and exhibit dominant clockwise rotation in vitro. We further show that cells in the developing myocardium are chiral as evident by a rightward bias of cell alignment and a rightward polarization of the Golgi complex, correlating with the direction of cardiac tube rotation. In addition, there is an LR polarized distribution of N-cadherin and myosin II in the myocardium before the onset of cardiac looping. More interestingly, the reversal of cell chirality via activation of the protein kinase C signaling pathway reverses the directionality of cardiac looping, accompanied by a reversal in cellular biases on the cardiac tube. Our results suggest that myocardial cell chirality regulates cellular LR symmetry breaking in the heart tube and the resultant directionality of cardiac looping. Our study provides evidence of an intrinsic cellular chiral bias leading to LR symmetry breaking during directional tissue rotation in vertebrate development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.