ArticleClinical chemistry2019
Integrated Extreme Real-Time PCR and High-Speed Melting Analysis in 52 to 87 Seconds.
Article in Clinical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Making Molecular Diagnostics Faster.International journal of laboratory hematology · 2026Review
- Advances in Genotyping Detection of Fragmented Nucleic Acids.Biosensors · 2024Review
- Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification.Micromachines · 2023Article
- Rapid Cycle and Extreme Polymerase Chain Reaction.Methods in molecular biology (Clifton, N.J.) · 2023Article
- World Dengue Day: A call for action.PLoS neglected tropical diseases · 2022Article
- Article
- Highly multiplex PCR assays by coupling the 5'-flap endonuclease activity ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Nearest-neighbour transition-state analysis for nucleic acid kinetics.Nucleic acids research · 2021Article
- First Movers in Molecular Detection: Case Comparison on Harnessing Research and Development, Industry, and Entrepreneurship.Frontiers in medicine · 2021Article
- Accurate, rapid and low-cost diagnosis of Mycoplasma pneumoniae via fast narrow-thermal-cycling denaturation bubble-mediated strand exchange amplification.Analytical and bioanalytical chemistry · 2020Article
- The vision of point-of-care PCR tests for the COVID-19 pandemic and beyond.Trends in analytical chemistry : TRAC · 2020Review
- Rapid detection and genotyping of ALK fusion variants by adapter multiplex PCR and high-resolution melting analysis.Laboratory investigation; a journal of technical methods and pathology · 2020Article
- The kinetic requirements of extreme qPCR.Biomolecular detection and quantification · 2019Article
- Extreme PCR Meets High-Speed Melting: A Step Closer to Molecular Diagnostics "While You Wait".Clinical chemistry · 2019Article
Corrections and comments
- Commented on by
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExtreme PCR in <30 s and high-speed melting of PCR products in <5 s are recent advances in the turnaround time of DNA analysis. Previously, these steps had been performed on different specialized instruments. Integration of both extreme PCR and high-speed melting with real-time fluorescence monitoring for detection and genotyping is presented here.
methodsA microfluidic platform was enhanced for speed using cycle times as fast as 1.05 s between 66.4 °C and 93.7 °C, with end point melting rates of 8 °C/s. Primer and polymerase concentrations were increased to allow short cycle times. Synthetic sequences were used to amplify fragments of hepatitis B virus (70 bp) and
resultsStandard rapid-cycle PCR chemistry did not produce any product when total cycling times were reduced to <1 min. However, efficient amplification was possible with increased primer (5 μmol/L) and polymerase (0.45 U/μL) concentrations. Infectious targets were amplified and identified in 52 to 71 s. Real-time PCR and genotyping of single-nucleotide variants from human DNA was achieved in 75 to 87 s and was 100% concordant to known genotypes.
conclusionsExtreme PCR with high-speed melting can be performed in about 1 min. The integration of extreme PCR and high-speed melting shows that future molecular assays at the point of care for identification, quantification, and variant typing are feasible.
Indexed as
Identifiers
30459167What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.