ArticlePloS one2018
Automated sequential chromogenic IHC double staining with two HRP substrates.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- From Stains to Spectra: Multiplexed Immunohistochemistry and Mass Spectrometry Imaging for Spatial Proteomics in Biomedicine.Advances in experimental medicine and biology · 2026Review
- Pentachroma O-H: A Five-Color Histological Staining Method for Enhanced Intestinal Tissue Analysis.International journal of molecular sciences · 2025Article
- Chromogen-based double immunohistochemical detection of mitochondrial respiratory chain deficiencies in human brain tissue.Acta neuropathologica communications · 2025Article
- Noncovalent minimal assembly of exogenous histamine with hemin cofactor as a peroxidase-mimicking cooperative catalyst.iScience · 2022Article
- Assessment of Lung Eosinophils In Situ Using Immunohistological Staining.Methods in molecular biology (Clifton, N.J.) · 2021Article
- How to distinguish between different cell lineages sharing common markers using combinations of double in-situ-hybridization and immunostaining in avian embryos: CXCR4-positive mesodermal and neural crest-derived cells.Histochemistry and cell biology · 2021Article
- Use of dual-marker staining to differentiate between lung squamous cell carcinoma and adenocarcinoma.The Journal of international medical research · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Automated IHC double staining using diaminobenzidine and HRP Magenta is illustrated utilizing a new acidic block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the first part of the double staining.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.