ArticlePloS one2018
Spectral features of nuclear DNA in human sperm assessed by Raman Microspectroscopy: Effects of UV-irradiation and hydration.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Exploring Human Sperm Metabolism and Male Infertility: A Systematic Review of Genomics, Proteomics, Metabolomics, and Imaging Techniques.International journal of molecular sciences · 2025Pooled it
- Oxidative Stress, Sperm DNA Fragmentation, or Both? Optimizing Test Selection in Male Infertility Evaluation.Antioxidants (Basel, Switzerland) · 2026Review
- Complex in vitro fertilization laboratory factors affecting sperm DNA integrity: A narrative review.International journal of reproductive biomedicine · 2025Review
- Andrology laboratory techniques for micro-TESE/IVF/ICSI: a narrative review.Asian journal of andrology · 2025Review
- Surface-Enhanced Raman Spectroscopy on Gold Nanoparticle for Sperm Quality Discrimination.Molecules (Basel, Switzerland) · 2025Article
- Non-invasive applications of Raman spectroscopy in assisted reproduction.Frontiers in endocrinology · 2025Review
- DNA damage in preimplantation embryos and gametes: specification, clinical relevance and repair strategies.Human reproduction update · 2022Review
- Decoding the role of cytochrome c in metabolism of human spermatozoa by Raman imaging.Frontiers in cell and developmental biology · 2022Article
- Microfluidic Systems for Isolation of Spermatozoa from Testicular Specimens of Non-Obstructive Azoospermic Men: Does/Can It Improve Sperm Yield?Journal of clinical medicine · 2021Review
- Advances in sperm analysis: techniques, discoveries and applications.Nature reviews. Urology · 2021Review
- ["Practical spermiogram"-semen analysis according to WHO recommendations].Der Urologe. Ausg. A · 2021Article
- Application and Progress of Raman Spectroscopy in Male Reproductive System.Frontiers in cell and developmental biology · 2021Review
- High spatially sensitive quantitative phase imaging assisted with deep neural network for classification of human spermatozoa under stressed condition.Scientific reports · 2020Article
- Sperm DNA Integrity and Male Fertility in Farm Animals: A Review.Frontiers in veterinary science · 2020Review
- The problem with being choosy when it comes to sperm selection.Journal of assisted reproduction and genetics · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Raman Microspectroscopy represents an innovative tool for the assessment of sperm biochemical features otherwise undetectable by routine semen analysis. Previously, it was shown that induced DNA damage can be detected in smeared sperm by this technique. This novel readout may be of value for clinical settings especially if it can be transferred to living cells. Yet, starting with living sperms this study was carried-out using a variety of conditions to disclose the Raman features of sperm nuclei under different hydration conditions and UV exposure. Human sperm were immobilized and Raman spectra were obtained from individual sperm as repeated measurements. To create conditions with controlled DNA damage, sperm samples were exposed to ultraviolet light. Several media were used to evaluate their effect on Raman spectra in aqueous conditions. To substantiate differences between the experimental conditions, the spectra were analyzed by Principal Component Analysis. We observed that spectra of sperm nuclei obtained in different solutions showed a qualitatively unchanged spectral pattern showing the principal signals related to DNA. Evaluating the effect of ultraviolet light generated the finding that spectra representing DNA damage were only observed in dry conditions but not in aqueous medium. Thus, Raman microspectroscopy was successfully applied for sperm analysis in different conditions, among them in live spermatozoa in aqueous solution during the initial measurement, revealing the principle use of this technique. However, implementation of Raman spectroscopy as a technique for clinical sperm analysis and selection may be especially relevant when DNA evaluation can be established using live sperm.
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