Evidence map›Paper›PMID 30455676›Full record

ArticleFrontiers in microbiology2018

A Screening Method for the Isolation of Bacteria Capable of Degrading Toxic Steroidal Glycoalkaloids Present in Potato.

Rosanna C Hennessy, Niels O G Jørgensen, Carsten Scavenius, Jan J Enghild, Mathias Greve-Poulsen, Ole Bandsholm Sørensen, Peter Stougaard

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. The Combination of Lead andFrontiers in microbiology · 2020
    Article
  10. Draft Genome Sequences of Two Glycoalkaloid-DegradingMicrobiology resource announcements · 2019
    Article
  11. Draft Genome Sequence of a NovelMicrobiology resource announcements · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Rosanna C HennessyDepartment of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark.
Niels O G JørgensenDepartment of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark.
Carsten ScaveniusDepartment of Molecular Biology and Genetics - Protein Science, Aarhus University, Aarhus, Denmark.
Jan J EnghildDepartment of Molecular Biology and Genetics - Protein Science, Aarhus University, Aarhus, Denmark.
Mathias Greve-PoulsenKMC, Kartoffelmelcentralen, Amba, Brande, Denmark.
Ole Bandsholm SørensenKMC, Kartoffelmelcentralen, Amba, Brande, Denmark.
Peter StougaardDepartment of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark.
University of Copenhagen · DKAarhus University · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Potato juice, a by-product of starch processing, is a potential high-value food ingredient due to its high protein content. However, conversion from feed to human protein requires the removal of the toxic antinutritional glycoalkaloids (GAs) α-chaconine and α-solanine. Detoxification by enzymatic removal could potentially provide an effective and environmentally friendly process for potato-derived food protein production. While degradation of GAs by microorganisms has been documented, there exists limited knowledge on the enzymes involved and in particular how bacteria degrade and metabolize GAs. Here we describe a series of methods for the isolation, screening, and selection of GA-degrading bacteria. Bacterial cultures from soils surrounding greened potatoes, including the potato peels, were established and select bacterial isolates were studied. Screening of bacterial crude extracts for the ability to hydrolyze GAs was performed using a combination of thin layer chromatography (TLC), high performance liquid chromatography (HPLC), and liquid chromatography mass spectrometry (LC-MS). Analysis of the 16S rRNA sequences revealed that bacteria within the genus

Indexed as

Arthrobacterglycoalkaloidsmicrobial enzymespotato fruit juicesoil bacteriaα-chaconineα-solanine

Identifiers

PMID30455676
PMCPMC6230958
OpenAlexW2900345772

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.