ArticleBiophysical journal2018
Adhesion of Active Cytoskeletal Vesicles.
Article in Biophysical journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many.ACS synthetic biology · 2023Review
- Differential regulation of GUV mechanics via actin network architectures.Biophysical journal · 2023Article
- Branched actin cortices reconstituted in vesicles sense membrane curvature.Biophysical journal · 2023Article
- Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex.Communications biology · 2023Article
- Identification of biomarker candidates for filarial parasite infections by analysis of extracellular vesicles.Frontiers in parasitology · 2023Article
- Methods to mechanically perturb and characterize GUV-based minimal cell models.Computational and structural biotechnology journal · 2023Review
- Studying actin-induced cell shape changes using Giant Unilamellar Vesicles and reconstituted actin networks.Biochemical Society transactions · 2022Review
- Femtoliter Injection of ESCRT-III Proteins into Adhered Giant Unilamellar Vesicles.Bio-protocol · 2022Article
- Vesicles Balance Osmotic Stress with Bending Energy That Can Be Released to Form Daughter Vesicles.The journal of physical chemistry letters · 2022Article
- Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture.Communications biology · 2021Article
- Reconstitution of contractile actomyosin rings in vesicles.Nature communications · 2021Article
- The ESCRT-III machinery participates in the production of extracellular vesicles and protein export during Plasmodium falciparum infection.PLoS pathogens · 2021Article
- Confinement Geometry Tunes Fascin-Actin Bundle Structures and Consequently the Shape of a Lipid Bilayer Vesicle.Frontiers in molecular biosciences · 2020Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulation of adhesion is a ubiquitous feature of living cells, observed during processes such as motility, antigen recognition, or rigidity sensing. At the molecular scale, a myriad of mechanisms are necessary to recruit and activate the essential proteins, whereas at the cellular scale, efficient regulation of adhesion relies on the cell's ability to adapt its global shape. To understand the role of shape remodeling during adhesion, we use a synthetic biology approach to design a minimal experimental model, starting with a limited number of building blocks. We assemble cytoskeletal vesicles whose size, reduced volume, and cytoskeletal contractility can be independently tuned. We show that these cytoskeletal vesicles can sustain strong adhesion to solid substrates only if the actin cortex is actively remodeled significantly. When the cytoskeletal vesicles are deformed under hypertonic osmotic pressure, they develop a crumpled geometry with deformations. In the presence of molecular motors, these deformations are dynamic in nature, and the excess membrane area generated thereby can be used to gain adhesion energy. The cytoskeletal vesicles are able to attach to the rigid glass surfaces even under strong adhesive forces just like the cortex-free vesicles. The balance of deformability and adhesion strength is identified to be key to enable cytoskeletal vesicles to adhere to solid substrates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.