Evidence map›Paper›PMID 30453211›Full record

ArticleVirology2019

Localization of the kinase Ataxia Telangiectasia Mutated to Adenovirus E4 mutant DNA replication centers is important for its inhibitory effect on viral DNA accumulation.

Dipendra Gautam, Gabrielle Stanley, Mary Owen, Eileen Bridge

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Dipendra GautamDepartment of Microbiology, Pearson Hall, Miami University, 700 E. High Street, Oxford, OH 45056, United States.
Gabrielle StanleyDepartment of Microbiology, Pearson Hall, Miami University, 700 E. High Street, Oxford, OH 45056, United States.
Mary OwenDepartment of Microbiology, Pearson Hall, Miami University, 700 E. High Street, Oxford, OH 45056, United States.
Eileen BridgeDepartment of Microbiology, Pearson Hall, Miami University, 700 E. High Street, Oxford, OH 45056, United States; Cell Molecular and Structural Biology Program, Miami University, Oxford, OH 45056, United States. Electronic address: BridgeE@MiamiOH.edu.
Pearson (United States) · US

Funding

NUCLEAR ORGANIZATION DURING ADENOVIRUS INFECTIONR15CA082111 · NCI · MIAMI UNIVERSITY OXFORD · PI BRIDGE, EILEEN K · 1999 to 2015
$1.0M
NCI NIH HHS R15 CA082111
6 · The paper itself

Abstract

Adenovirus (Ad) type 5 (Ad5) E4 deletion mutants including H5dl1007 (E4-) induce a DNA damage response (DDR) that activates the kinase ataxia-telangiectasia mutated (ATM), which can interfere with efficient viral DNA replication. We find that localization of active phosphorylated ATM (pATM) to E4- viral replication centers (VRCs) is important for its inhibitory effect. ATM is necessary for localization of RNF8 and 53BP1 to E4 mutant VRCs, while recruitment of DDR factors Mre11, Mdc1 and γH2AX is ATM-independent, raising the possibility that ATM may affect viral chromatin at VRCs. We assessed E4- and Ad5 chromatin organization by micrococcal nuclease (MN) digestion. A significant fraction of Ad5 DNA is somewhat resistant to MN digestion, whereas E4- DNA is more susceptible. ATM inhibition increases the fraction of E4- DNA that is resistant to MN digestion. Our results address possible mechanisms through which ATM inhibits E4- DNA replication.

Indexed as

Virus ReplicationAdenoviridaeAdenoviridae InfectionsAdenovirus E4 ProteinsAtaxia Telangiectasia Mutated ProteinsCell LineChromatinDNA-Binding ProteinsDNA RepairDNA, ViralGene DeletionHumansMicrococcal NucleaseMultiprotein ComplexesPhosphorylationTumor Suppressor p53-Binding Protein 1Adenovirus E4 ProteinsAtaxia Telangiectasia Mutated ProteinsChromatinDNA-Binding ProteinsDNA, ViralMicrococcal NucleaseMultiprotein ComplexesRNF8 protein, humanTP53BP1 protein, humanTumor Suppressor p53-Binding Protein 1Ubiquitin-Protein Ligases53BP1AdenovirusATMDNA damage responseE4 11 kDaE4 34 kDaE4 ORF 3E4 ORF 6Micrococcal nucleaseRNF8

Identifiers

PMID30453211
PMCPMC6874311
OpenAlexW2901649842

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.