ArticlePoultry science2019
The impact of Bacillus subtilis DSM 32315 on the pathology, performance, and intestinal microbiome of broiler chickens in a necrotic enteritis challenge.
Article in Poultry science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
49 citing papers in PubMed, 93 citations in OpenAlex.
- Effects of replacing antibiotics with probiotics and antimicrobial peptides on performance, gut health, carcass traits, meat quality, and welfare in broilers infected with Eimeria and Clostridium perfringens.Tropical animal health and production · 2025Article
- Winning the battle of intestinal peace withFrontiers in microbiology · 2025Review
- Performance and Health Parameters of Sows and Their Litters Using a Probiotic Supplement Composed ofAnimals : an open access journal from MDPI · 2024Article
- Dietary administration of Bacillus subtilis improves the health parameters and regulates the gene expression in mice receiving zearalenone-contaminated diet.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Dietary Supplementation of Brevibacillus laterosporus S62-9 Improves Broiler Growth and Immunity by Regulating Cecal Microbiota and Metabolites.Probiotics and antimicrobial proteins · 2024Article
- Effects of fermented sweet potato residue on nutrient digestibility, meat quality, and intestinal microbes in broilers.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- A comprehensive review of experimental models and induction protocols for avian necrotic enteritis over the past 2 decades.Frontiers in veterinary science · 2024Review
- Solitary Bees Host More Bacteria and Fungi on Their Cuticle than Social Bees.Microorganisms · 2023Article
- Bacillus subtilis, a Swiss Army Knife in Science and Biotechnology.Journal of bacteriology · 2023Review
- Recent Trends on Mitigative Effect of Probiotics on Oxidative-Stress-Induced Gut Dysfunction in Broilers under Necrotic Enteritis Challenge: A Review.Antioxidants (Basel, Switzerland) · 2023Review
- ProbioticMicroorganisms · 2023Article
- Fungicides and insecticides can alter the microbial community on the cuticle of honey bees.Frontiers in microbiology · 2023Article
- Effects ofFrontiers in physiology · 2023Article
- The impact ofFrontiers in veterinary science · 2023Article
- Probiotics as Alternatives to Antibiotics for the Prevention and Control of Necrotic Enteritis in Chickens.Pathogens (Basel, Switzerland) · 2022Review
- Effects of zinc and Bacillus subtilis on the reproductive performance, egg quality, nutrient digestion, intestinal morphology, and serum antioxidant capacity of geese breeders.Poultry science · 2022Article
- Effects of dietary glucose oxidase on growth performance and intestinal health of AA broilers challenged by Clostridium perfringens.Poultry science · 2022Article
- Review
- DietaryFrontiers in veterinary science · 2022Article
- Novel strategies to improve chicken performance and welfare by unveiling host-microbiota interactions through hologenomics.Frontiers in physiology · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It was hypothesized that dietary inclusion of Bacillus subtilis DSM 32315 could inhibit Clostridium perfringens induced necrotic enteritis (NE), thereby improving broiler performance. Male, d 0 chicks were randomly assigned 14 birds/pen, 11 pens/treatment in 3 treatments: a basal diet (control), a coccidiostat fed control (Narasin), and a direct fed microbial (DFM) B. subtilis DSM 32315 treatment. Necrotic enteritis was induced in all birds by oral inoculation of Eimeria maxima oocysts on d 12 and a virulent C. perfringens on d 16. Mortality was reduced (P < 0.001) in DFM and Narasin compared to control. DFM reduced (P < 0.001) feed conversion ratio (FCR) compared to control. Furthermore, DFM and Narasin reduced (P < 0.001) footpad lesions. The DFM was shown to increase (P < 0.05) Bacillus spp. and decrease (P < 0.05) C. perfringens in the ileum and cecum at several time points. To investigate microbiome changes in the cecum, digesta samples were analyzed with % guanine and cytosine (%G+C) microbial profiling which fractionates bacterial chromosomes based on the %G+C in DNA. The method revealed treatment profile peaks in low (27.0 to 34.5%), mid (40.5 to 54.0%), and high (59.0 to 68.0%) G+C fractions. 16S rRNA gene amplification and high throughput sequencing was conducted on each of these fractions in order to elucidate specific bacterial population differences. In the low and mid %G+C fractions, DFM had greater abundance of Lactobacillaceae family members (P = 0.03 and P = 0.01, respectively) and Lactobacillus salivarius (P = 0.04 and P = 0.01, respectively) than control or Narasin. Lactobacillus johnsonii was also greater in the low %G+C fraction compared to control and Narasin (P = 0.01). Lachnospiraceae (P = 0.04) and Ruminococcaceae (P < 0.01) in the mid %G+C fraction were reduced in the DFM compared to control. Positive alterations to the microbial populations in the gut of broilers may at least be a partial mechanism by which B. subtilis DSM 32315 reduced pathology and improved performance of broilers in the NE challenge.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.