ArticlePLoS neglected tropical diseases2018
Vertical transmission of naturally occurring Bunyamwera and insect-specific flavivirus infections in mosquitoes from islands and mainland shores of Lakes Victoria and Baringo in Kenya.
Article in PLoS neglected tropical diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
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- Leveraging insect-specific viruses to elucidate mosquito population structure and dynamics.PLoS pathogens · 2023Review
- Analyses of Mosquito Species Composition, Blood-Feeding Habits and Infection with Insect-Specific Flaviviruses in Two Arid, Pastoralist-Dominated Counties in Kenya.Pathogens (Basel, Switzerland) · 2023Article
- Composition and global distribution of the mosquito virome - A comprehensive database of insect-specific viruses.One health (Amsterdam, Netherlands) · 2023Article
- A tangled threesome: understanding arbovirus infection inFrontiers in microbiology · 2023Review
- Deciphering the Tissue Tropism of the RNA Viromes Harbored by Field-Collected Anopheles sinensis and Culex quinquefasciatus.Microbiology spectrum · 2022Article
- Vertical and Horizontal Transmission of Cell Fusing Agent Virus in Aedes aegypti.Applied and environmental microbiology · 2022Article
- Molecular screening reveals non-uniform malaria transmission in western Kenya and absence of Rickettsia africae and selected arboviruses in hospital patients.Malaria journal · 2022Article
- Vector competence and immune response of Aedes aegypti for Ebinur Lake virus, a newly classified mosquito-borne orthobunyavirus.PLoS neglected tropical diseases · 2022Article
- Jingmen Tick Virus in Ticks from Kenya.Viruses · 2022Article
- Evidence of circulation of Orthobunyaviruses in diverse mosquito species in Kwale County, Kenya.Virology journal · 2021Article
- Tick-borne pathogens, including Crimean-Congo haemorrhagic fever virus, at livestock markets and slaughterhouses in western Kenya.Transboundary and emerging diseases · 2021Article
- Symbiotic Interactions Between Mosquitoes and Mosquito Viruses.Frontiers in cellular and infection microbiology · 2021Review
- A survey of mosquito-borne and insect-specific viruses in hospitals and livestock markets in western Kenya.PloS one · 2021Article
- Diversity and Abundance of Potential Vectors of Rift Valley Fever Virus in the North Region of Cameroon.Insects · 2020Article
- Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses.PLoS neglected tropical diseases · 2020Article
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6 authors at 1 institution in 2 countries.
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Abstract
backgroundMany arboviruses transmitted by mosquitoes have been implicated as causative agents of both human and animal illnesses in East Africa. Although epidemics of arboviral emerging infectious diseases have risen in frequency in recent years, the extent to which mosquitoes maintain pathogens in circulation during inter-epidemic periods is still poorly understood. This study aimed to investigate whether arboviruses may be maintained by vertical transmission via immature life stages of different mosquito vector species. METHODOLOGY: We collected immature mosquitoes (egg, larva, pupa) on the shores and islands of Lake Baringo and Lake Victoria in western Kenya and reared them to adults. Mosquito pools (≤25 specimens/pool) of each species were screened for mosquito-borne viruses by high-resolution melting analysis and sequencing of multiplex PCR products of genus-specific primers (alphaviruses, flaviviruses, phleboviruses and Bunyamwera-group orthobunyaviruses). We further confirmed positive samples by culturing in baby hamster kidney and Aedes mosquito cell lines and re-sequencing. PRINCIPAL
findingsCulex univittatus (2/31pools) and Anopheles gambiae (1/77 pools) from the Lake Victoria region were positive for Bunyamwera virus, a pathogenic virus that is of public health concern. In addition, Aedes aegypti (3/50), Aedes luteocephalus (3/13), Aedes spp. (2/15), and Culex pipiens (1/140) pools were positive for Aedes flaviviruses at Lake Victoria, whereas at Lake Baringo, three pools of An. gambiae mosquitoes were positive for Anopheles flavivirus. These insect-specific flaviviruses (ISFVs), which are presumably non-pathogenic to vertebrates, were found in known medically important arbovirus and malaria vectors.
conclusionsOur results suggest that not only ISFVs, but also a pathogenic arbovirus, are naturally maintained within mosquito populations by vertical transmission, even in the absence of vertebrate hosts. Therefore, virus and vector surveillance, even during inter-epidemics, and the study of vector-arbovirus-ISFV interactions, may aid in identifying arbovirus transmission risks, with the potential to inform control strategies that lead to disease prevention.
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